CHARACTERIZATION OF PSEUDOMONAS AERUGINOSA PILI GENES

铜绿假单胞菌菌毛基因的特征

• 批准号: 3436629
• 负责人: PETER A. CASTRIC
• 金额: $ 6.79万
• 依托单位: DUQUESNE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-30 至 1989-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3436629
• 关键词: Escherichia coli; Pseudomonas aeruginosa; autoradiography; bacterial antigens; bacterial genetics; gel electrophoresis; gene expression; genes; genetic manipulation; genetic mapping; genetic markers; genetic transcription; molecular cloning; mutant; pilus; plasmids; point mutation; surface antigens; transposon /insertion element; virulence

The proposed work will study the genes involved in synthesis and assembly of the Pseudomonas aeruginosa 1244 pilus, a major surface antigen and virulence factor. These genes will be marked using transposon mutagenesis and will be analyzed as to phenotype. The transposon marker will be cloned from mutants unable to assemble pili and will be analyzed as to physical structure. Minicell studies of these and wild type will be used to identify number, size, relative position, direction of transcription, and polarity of these genes, as well as final subcellular location of the gene product. This information will be used to identify potential antigens for vaccine evaluation.

这项拟议的工作将研究与合成有关的基因。 和组装铜绿假单胞菌1244菌毛，一个主要的 表面抗原和毒力因子。这些基因将是 用转座子突变标记的，将被分析为 表型。转座子标记将从突变体中克隆出来 无法组装菌毛，将根据物理特性进行分析 结构。对这些和野生型的Minicell研究将是 用于标识数量、大小、相对位置、方向 转录，这些基因的极性，以及最终的 基因产物的亚细胞定位。此信息 将被用来识别疫苗的潜在抗原 评估。

项目成果

Cloning and sequencing of the Pseudomonas aeruginosa 1244 pilin structural gene.

铜绿假单胞菌 1244 菌毛蛋白结构基因的克隆和测序。

• DOI: 10.1007/bf00332233
• 发表时间: 1989
• 期刊: Molecular & general genetics : MGG
• 作者: Castric,PA;Sidberry,HF;Sadoff,JC
• 通讯作者: Sadoff,JC