THE EVALUATION AND BANKING OF ABORTED FETAL TISSUES

我们建议建立一个大型的、全面的胎盘和胎儿资料库 来自自然流产和宫外孕的样本， 包括详细的临床数据(尽可能包括亲代样本)。 胎儿和胎盘组织(以及从它们中提取的RNA或DNA)将 对于我们正在进行的早期妊娠丢失原因的研究来说，这是必不可少的。 最终，收集的材料可能会被证明是一个有价值的来源 用于胎儿组织移植的干细胞(特别是在犹他州 相对较少的怀孕是“选择性”终止的)。我们的 多学科团队将详尽地研究 已知和疑似病因的患病率和意义银行 各种因素。药物召回病史和尿毒学筛查将 从每一个同意的女人那里得到的。胚胎和胎儿组织将被 对染色体异常的分析将进行详细的 畸形学/畸形学检查，并将进行相关培养 细菌和病毒。DNA扩增技术也将被用于 一种对组织进行微生物筛选的免费方法。 多态DNA标记将用于比较母亲、父亲和 胎儿样本用于筛查通常不明显的遗传事件 常规细胞遗传学检查(如同体、微缺失和 单基因突变)最后，我们将测试获得 并用来自中国的胎儿肝脏培养胎儿造血祖细胞 自然流产和宫外孕。……的成败 这些培养将与我们在培养新生儿方面的成功进行比较 造血祖细胞，并将与临床数据和 实验室调查。延长干细胞的可获得性 对于移植，我们将尝试长期培养和冷冻保存 造血祖的基因。

We propose to develop a large, comprehensive bank of placental and fetal specimens from spontaneous abortions and ectopic pregnancies, which includes detailed clinical data (and parental specimens whenever possible). Fetal and placental tissues (and RNA or DNA extracted from them) will be essential for our ongoing research into the causes of early pregnancy loss. Ultimately, the material collected may prove to be a valuable source of stem cells for fetal tissue transplantation (especially in Utah where relatively few pregnancies are terminated "electively"). Our multi-disciplinary team will exhaustively characterize specimens in the bank for the prevalence and significance of known and suspected etiologic factors. A drug recall history and a urine toxicology screen will be obtained from every consenting woman. Embryonic and fetal tissues will be analyzed for chromosomal abnormalities will undergo a detailed teratology/dysmorphology examination, and will be cultured for relevant bacteria and viruses. DNA amplification techniques will also be used as a complimentary approach to screen the tissues for microorganisms. Polymorphic DNA markers will be used to compare maternal, paternal, and fetal samples to screen for genetic events which are not normally apparent on routine cytogenetic examination (such as isodisomy, microdeletions, and single gene mutations) Finally, we will test the feasibility of obtaining and culturing fetal hematopoietic progenitors using fetal liver from spontaneous abortions and ectopic pregnancies. The success or failure of these cultures will be compared with our success in culturing neonatal hematopoietic progenitors and will be correlated with the clinical data and the laboratory investigations. To prolong their availability of stem cells for transplantation, we will attempt long-term culture and cryopreservation of the hematopoietic progenitors.