THE STUDY OF CELL JUNCTIONS IN THE OCULAR LENS

我们拟研究大鼠、兔和兔眼晶状体的细胞连接， 青蛙 研究计划的具体目标包括：（1）建立一个比较 林隙结构特征与分布格局研究 大鼠、兔和青蛙正常透镜纤维连接;（2）研究可能 透镜纤维间隙连接的结构改变 大鼠半乳糖性白内障的发生和逆转;（3）a 缝隙连接结构特征的比较研究 大鼠、兔和青蛙正常透镜上皮细胞;（4）研究 上皮细胞间隙连接的结构发生差异， 大鼠半乳糖性白内障的发生和逆转;（5）研究 分化区缝隙连接的形成模式 半乳糖性白内障发展和逆转过程中的上皮;（6） 研究其结构特征和生理渗透性 蛙、大鼠透镜上皮细胞的紧密连接 （7）测定与细胞凋亡相关的缝隙连接结构的变化。 血清激活上皮细胞分裂和生长 兔透镜器官培养。 所涉方法如下： 冷冻断裂TEM（常规和快速冷冻技术）， 电子显微镜;注射和孵育血红素蛋白示踪剂， 细胞化学和器官培养技术等。 调查的总体意义在于：（1）提供 更好地理解缝隙连接的结构特征 紧密连接和生理渗透性 不同种属晶状体中的闭锁小带，（2）显示可能 与白内障发生相关的缝隙连接的变化，（3） 确定缝隙连接在细胞调控中的可能作用 器官培养兔透镜上皮细胞的增殖和生长。

We propose to study cell junctions in the ocular lenses of rat, rabbit and frog. The specific aims of the research plan include: (1) A comperative study of the structural characteristics and distribution pattern of gap junctions in normal lens fiber of rat, rabbit and frog; (2) study possible structural alterations of lens fiber gap junctions associated with the development and reversal of galactose cataract in the rat; (3) a comparative study of the structural characteristics of gap junctions in normal lens epithelia of rat, rabbit and frog; (4) study whether differences occur in the structure of epithelial gap junctions during the development and reversal of galactose cataract in the rat; (5) study formation pattern of gap junctions in the zone of differentiating epithelium during development and reversal of galactose cataracts; (6) study the structural characteristics and physiological permeability of zonulae occludentes (tight junctions) in the lens epithelia of frog, rat and rabbit; (7) determine the changes in gap junction structure related to the activation of cell division and growth of epithelial cells by serum in rabbit lens organ culture. Methodology involved will be as follows: Freeze-fracture TEM (conventional and rapid freezing techniques), light and electron microscopy; injection and incubation of hemeprotein tracers, cytochemistry, and organ culture techniques etc. The overall significance of the investigation is that it may: (1) provide a better understanding of the structural characteristics of gap junctions and zonulae accludentes (tight junctions), and physiological permeability of zonulae occludentes in lenses of various species, (2) show possible changes in gap junctions that are related to cataractogenesis, (3) determine the possible role of gap junctions in the control of cell proliferation and growth of the rabbit lens epithelium in organ culture.