THE EVALUATION OF LASER RADIATION ON ATHEROSCLEROSIS

该项目的目标将是研究当前和长期的 激光照射对动脉粥样硬化的有利或不利影响 各种动物模型的斑块和正常动脉。所完成的工作 首席研究员已经证明了使用激光的可行性 适用于光纤导管输送系统以重建新的 完全闭塞的人体尸检冠状动脉中的血管通道。 这是在血液或生理盐水介质下完成的。另外，初步的 在活体兔动脉粥样硬化模型中的工作继续证明 通过光纤系统传输的激光的能力 对动脉粥样硬化阻塞的动脉进行再灌注术。激光对人体的伤害 斑块是一个干净的伤口，在动脉的横截面上显示 斑块被分成楔形的部分。血管造影术没有发现任何 兔远端血管激光照射后的碎片 动脉粥样硬化模型。进一步测试远端栓塞术是否会 发生在激光照射后的狗模型使用移植的人 将对动脉粥样硬化的冠状动脉进行评估。过滤器将是 放置在激光场地的远端，以收集碎片。在其他狗身上(有 正常动脉)将进行长期研究，以评估其潜力 激光辐射对血管壁的有害影响，即动脉瘤， 穿孔，血栓形成，超过一个月的时间。这些船只将 通过光学和电子显微镜技术进行研究。其他内容 将进行实验来研究激光辐射的长期影响。 在动脉粥样硬化猴子的血管上。这些船只将通过以下机构进行评估 光学显微镜和电子显微镜。在所有这些实验中， 使用的激光能量(瓦特X时间)将被记录在顺序中，以确定 在不生产的情况下对阻塞的血管进行再灌注化的最佳范围 严重的脉管壁损伤。

The objective of this project will be to study the immediate and long term beneficial or detrimental effects of laser radiation on the atheroslcerotic plaque and normal arteries in various animal models. Work done by the principal investigator has demonstrated the feasibility of using lasers adapted to a fiberoptic-catheter delivery system to re-establish new vascular channels in totally occluded human necropsy coronary arteries. This was accomplished under a blood or saline media. Also, preliminary work in the living rabbit atherosclerotic model continues to demonstrate the ability of the laser delivered through a fiberoptic system to recannalize arteries obstructed by atherosclerosis. The laser injury to plaque is a clean incision which on cross section of the artery shows the plaque divided into wedge-shaped segments. Angiography did not reveal any debris in the distal vessels following laser radiation of the rabbit atherosclerotic model. To further test whether distal embolization will occur following laser radiation a dog model using transplanted human atherosclerotic coronary arteries will be evaluated. Filters will be placed distally to the lased site to collect debris. In other dogs (with normal arteries) long term studies will be done to evaluate the potential detrimental effects of laser radiation on the vessel wall, i.e., aneurysm, perforation, thrombosis, over a one month period of time. The vessels will be studied by light and electron microscopic techniques. Additional experiments will be done to study the long term effects of laser radiation on vessels of atherosclerotic monkeys. These vessels will be evaluated by light and electron microscopy. In all of these experiments, the amount of laser energy (watts X time) used will be recorded in rder to determine optimal ranges to recannalize obstructed vessels without producing significant vessel wall damage.