SERTOLI CELL-GERM CELL SURFACE MEMBRANE INTERACTIONS

这个应用中的实验开始检查细胞表面 支持细胞和生精细胞之间的相互作用 在精子发生过程中，通过表征生殖细胞与 支持细胞体外培养。这种支持细胞与生殖细胞的结合作用 将被描述为五个具体目标的实现。(1)在 将开发体外试验来评估和量化特定的 生殖细胞与支持细胞的结合。(2)抗肿瘤的单抗 将生成支持细胞表面组件并从功能上进行选择 基于它们抑制生殖细胞与Sertoli结合的能力 细胞。(3)与细菌结合有关的支持细胞表面抗原 细胞将使用所选的单抗进行鉴定。(4) 具有生殖细胞结合亲和力的支持细胞表面抗原的表达 将会被研究。这一表达依赖于细菌的存在 将检查细胞以及对这种表达的调节 卵泡刺激素和睾丸素。(五)关系 Sertoli细胞表面抗原和分泌产物之间的关系 检查过了。 支持细胞与生精细胞的细胞表面相互作用 很可能有助于控制雄性减数分裂和有序 减数分裂后细胞的分化。特殊识别和粘合 涉及细胞表面的事件可能先于GAP的后续发展 支持细胞和精母细胞之间的连接。类似地，支持细胞 协调减数分裂后管腔运动的表面特化 通过生精上皮的细胞可能依赖于先前的细胞 认可度和粘合度。Sertoli的表面分子 细胞-生精细胞的识别和结合可能占据关键的 精子细胞发育的控制点。支持细胞的研究 负责正常细胞识别和结合的表面分子 生精细胞将有助于确定这些细胞的不适当活动 表面分子导致精子产生异常的条件 如果这些分子的活性能够被调节以减少精子 在需要的时候进行生产。

The experiments in this application begin to examine cell surface interactions between Sertoli cells and spermatogenic cells that occur during spermatogenesis by characterizing the binding of germ cells to Sertoli cells in vitro. This Sertoli cell-germ cell binding interaction will be characterized as five specific aims are accomplished. (1) An in vitro assay will be developed to evaluate and quantitate the specific binding of germ cells to Sertoli cells. (2) Monoclonal antibodies to Sertoli cell surface components will be generated and functionally selected on the basis of their ability to inhibit germ cell binding to Sertoli cells. (3) The Sertoli cell surface antigens involved in binding germ cells will be identified using the monoclonal antibodies selected. (4) The expression of Sertoli cell surface antigens with germ cell binding affinity will be studied. The dependence of this expression on the presence of germ cells will be examined as well as the regulation of this expression by follicle-stimulating hormone and by testosterone. (5) The relationship between Sertoli cell surface antigens and secretory products will be examined. Cell surface interactions between Sertoli cells and spermatogenic cells very likely contribute to the control of male meiosis and to the orderly differentiation of post-meiotic cells. Specific recognition and adhesive events involving the cell surface may precede subsequent development of gap junctions between Sertoli cells and spermatocytes. Similarly, Sertoli cell surface specializations that coordinate lumenal movement of post-meiotic cells through the seminiferous epithelium may depend on prior cell recognition and adhesion. The surface molecules responsible for Sertoli cell-spermatogenic cell recognition and binding may occupy a critical control point in sperm cell development. The study of the Sertoli cell surface molecules responsible for normal cell recognition and binding of spermatogenic cells will help determine if inappropriate activity of these surface molecules contributes to conditions of abnormal sperm production and if the activity of these molecules can be modulated to decrease sperm production when desired.