EFFECT OF ZINC AND COPPER IMBALANCE ON SERUM LIPIDS

拟议研究的主要目标是研究 口服锌补充剂对血清甘油三酯、总胆固醇和 年轻白色人的脂蛋白-胆固醇分数和血压 男性。 次要目的是研究口服锌的效果 补充血清、头发和尿锌和铜浓度。 三组白色男性（每组12-15人），年龄18-29岁，将 接受50 mg硫酸锌形式的锌，75 mg硫酸锌形式的锌，或 明胶胶囊，12周。 受试者将不知道 他们正在接受的治疗。 三天饮食记录将由以下人员完成： 受试者开始补充后每两周一次。 食品样品也将 在同一时期收集。 饮食记录和食物样本将 分析每日摄入的卡路里，蛋白质，碳水化合物，脂肪， 多不饱和：饱和脂肪的比例，以及铜和锌的摄入量。 将采集毛发、24小时尿液样本和过夜空腹血清样本， 在补充前和不同时间从每个受试者中采集 之后的间隔。 头发，尿液，和血清将分析铜 和锌浓度。 毛发和血清数据将以部分表示 而尿液数据将表示为ng Cu或Zn/gm 肌酸酐 食品样品也将分析铜，锌， 钙浓度。 水样也将进行分析， 元素 在整个研究期间，受试者将维持低血糖饮食 在铜、锌、植酸盐和纤维中，以避免锌的干扰 吸收 所有受试者的饮食史将在 研究的开始。 重复测量设计（裂区至 时间）将用于分析补锌对 因变量 年龄、体重/身高的协变调整， 卡路里的摄入量。 其他膳食摄入量数据 锌补充剂以外的其他成分也会影响结果。

The primary objective of the proposed research is to study the effect of oral zinc supplementation upon serum triglycerides, total cholesterol, and lipoprotein-cholesterol fractions, and blood pressure in young white males. A secondary objective is to study the effect of oral zinc supplementation upon serum, hair, and urine zinc and copper concentration. Three groups of white males (12-15 in each group), ages 18-29 years, will receive either 50 mg zinc as zinc sulfate, 75 mg zinc as zinc sulfate, or gelatin capsules, for twelve weeks. Subjects will be unaware of the treatment they are receiving. Three day diet records will be completed by subjects biweekly after supplementation begins. Food samples will also be collected at the same time periods. Diet records and food samples will be analyzed for daily intakes of calories, protein, carbohydrate, fat, polyunsaturated:saturated fat ratio, as well as copper and zinc intake. Hair, a 24 hr urine sample, and overnight fasting serum sample will be taken from each subject before supplementation and at various time intervals there-after. Hair, urine, and serum will be analyzed for copper and zinc concentrations. Hair and serum data will be expressed in parts per million, whereas urine data will be expressed as ng Cu or Zn per gm creatinine. Food samples will also be analyzed for copper, zinc, and calcium concentrations. Water samples will also be analyzed for the same elements. Throughout the study, subjects will be maintained on a diet low in copper, zinc, phytate, and fiber to avoid interferences in zinc absorption. Diet histories of all subjects will be collected at the beginning of the study. A repeated measures design (split plot through time) will be used to analyze the effect of zinc supplementation upon dependent variables. Covariant adjustment for age, weight/height, and calorie intake will be made. Data on the intake of other dietary components other than zinc supplementation are affecting results.