INTEGRATIVE CARDIORESPIRATORY CONTROL DURING EXERCISE

中枢指令和反射起源于工作的骨骼肌， 两种可能的控制机制负责心肺 对锻炼的反应。 尽管这两个假设已经被研究过了， 单独地，很少有人知道这两个控制之间的相互作用 机制等 因此，拟议的项目将试图位于中央 猫的中枢神经系统， 外围反馈 一系列实验将在 麻醉猫 首先，刺激引起的心肺反应 在Forel的底丘脑区域，一种被认为可以激活中枢神经系统的策略， 在此之前和之后，将确定在一个命令， 延髓部位（外侧网状核）被认为与 心血管对肌肉收缩的反射反应 下一个是 引起的肌肉收缩的心血管和舒张反应 将在消融前和消融后测量腹根刺激。 Forel的田野 最后，Forel刺激和腹根 在损伤之前和之后将分别进行刺激， 小脑区（小脑顶核）参与心肺 对锻炼的反应。 在第二个系列的实验中，猫将 用于慢性心血管测量的仪器，然后经过培训， 做等长运动。 这些有意识的猫将在 并且在进行与在麻醉的猫中进行的损伤相同的损伤之后。 在最后一系列的实验中，微电极记录将在 麻醉猫，以确定是否Forel，小脑顶核或 外侧网状核具有适当的神经连接， 中央指令和运动肌肉反馈的整合。 是 希望这些实验能够识别出 将下行中央命令与外围反馈相结合， 对锻炼的适当反应。 这些知识可能有助于 临床医生有神经功能缺损的患者， 对锻炼的反应。

Central command and a reflex originating in working skeletal muscles are two possible control mechanisms responsible for the cardiorespiratory responses to exercise. Even though these two hypotheses have been studied separately, little is known about interactions between these two control mechanisms. Therefore the proposed project will attempt to locate central neural sites in cats that are involved in both central command and peripheral feedback. One series of experiments will be performed in anesthetized cats. First, the cardiorespiratory responses to stimulation in the subthalamic fields of Forel, a maneuver thought to activate central command, will be determined before and after lesions are placed in a medullary site (the lateral reticular nucleus) believed to be associated with the reflex cardiovascular responses to muscular contraction. Next, cardiovascular and ventilatory responses to muscular contraction induced by ventral root stimulation will be measured before and after ablation of the fields of Forel. Finally, fields of Forel stimulation and ventral root stimulation will be performed separately before and after lesioning a cerebellar area (the fastigial nucleus) involved in the cardiorespiratory responses to exercise. In a second series of experiments, cats will be instrumented for chronic cardiovascular measurements and then trained to perform isometric exercise. These conscious cats will be studied before and after making lesions identical to those made in the anesthetized cats. In a final series of experiments, microelectrode recordings will be made in anesthetized cats to establish if the fields of Forel, fastigial nucleus or the lateral reticular nucleus have appropriate neural connections for integration of central command and feedback from exercising muscles. It is hoped that these experiments will identify central neural sites that integrate descending central command with peripheral feedback to provide the appropriate responses to exercise. This knowledge may be useful to clinicians having patients with neurological deficits causing abnormal responses to exercise.