CELL BIOLOGY IMMUNOLOGY ON ANIMAL MODELS FOR VITILIGO

本研究的长期目标是 对发生在 白癜风是一种皮肤和眼睛的色素细胞 摧毁. 这些实验旨在分析 在两种白癜风动物模型中的色素脱失：广泛的 研究了史密斯鸡（以前称为DAM） 鸡）和一种新的哺乳动物鼠模型（vit/vit）。 两 要采取的方法涉及基本的黑素细胞缺陷， 随后的自身免疫反应。 猪和 免疫系统将不得不被协调地操纵， 任何可能由以下结果指示的治疗方案： 建议的研究。 这些实验包括深入研究黑化在 Smyth线，在脉络膜黑素细胞和神经管中 衍生的黑素细胞在培养中以一种 类似于在体内黑素细胞中观察到的。 此外，本发明还提供了一种方法， 试图纠正史密斯的异常行为 提出了体外和体内的黑素细胞。 休闲 异常黑化与选择性 还将研究黑素体的自体吞噬作用。 来自在各种不同时间段获得的史密斯鸡的血清 发展阶段的白癜风将筛选B 黑素细胞特异性抗体。 这些抗体将被用于 定位黑素细胞特异性抗原 通过电子显微镜免疫细胞化学。 搜索 皮肤与脉络膜黑素细胞特异性表面抗原 将通过建立各自的纯文化开始 黑素细胞类型。 此外，形态学和组织化学 Vit/Vit小鼠的毛球和眼睛色素脱失分析 以及抗黑素细胞因子的筛查 在这个过早变灰的突变品系的血清中， 黑素细胞培养物的发育。

The long-term objective of the research proposed here is to advance knowledge of the pathologic processes occurring in vitiligo, a disease in which pigment cells of the skin and eyes are destroyed. The experiments are aimed at analyzing the basis for depigmentation in two animal models for vitiligo: the extensively studied Smyth chicken (previously referred to as the DAM chicken) and a new mammalian, murine model (vit/vit). The two approaches to be taken concern the basic melanocytic defect and the subsequent autoimmune response. Both the pigmentary and the immune systems will have to be manipulated in concert during any therapeutic regimen that might be indicated by the results of the proposed research. The experiments include in-depth studies of melanization in the Smyth line, both within chororidal melanocytes and in neural-tube derived melanocytes that malfunction in culture in a manner similar to that observed in melanocytes in vivo. In addition, attempts to rectify the aberrant behavior of Smyth melanocytes, both in vitro and in vivo, are proposed. The casual relationship between aberrant melanization and the selective authophasgocytosis of melanosomes will also be investigated. Serum from Smyth chickens obtained during various developmental stages of the vitiligo will b screened for melanocyte-specific antibodies. Such antibodies will then be used to localize the melanocyte-specific antigens immunocytochemically by electron microscopy. A search for cutaneous versus choroidal melanocyte-specific surface antigens will be initiated by establishing pure cultures of the respective melanocyte types. In addition, morphologic and histochemical analysis of hair bulb and eye depigmentation in the vit/vit mouse will be initiated as well as screening for anti-melanocyte factors in the serum of this prematurely graying mutant line and development of melanocyte cultures.