PATCH CLAMP STUDIES OF MAMMALIAN RETINAL GLIAL CELLS

这项研究计划的长期目标是使用膜片钳 研究视网膜神经胶质细胞的病理生理学技术。知道 更多关于视网膜神经胶质细胞是可取的，因为这些细胞似乎是 与多种玻璃体视网膜疾病有关。一个特定的目标是使用 膜片钳技术研究生理学、药理学和组织化学 成年大鼠视网膜神经胶质细胞离子通道的调节 来自接受玻璃体切除手术的人类。初步调查结果表明， 大鼠视网膜神经胶质细胞至少具有两种离子通道。 实验还表明，检测神经胶质细胞中的离子通道是可行的 从病人身上获得的细胞。 有关视网膜神经胶质细胞离子通道的有限知识可能 是我们对这一疾病的病理生理学认识上的一个重要缺口 单元格的类别。离子通道可能在以下方面发挥重要作用 细胞在正常和病理生理条件下的活动。在… 目前，除初步研究外，尚无膜片钳研究 大鼠视网膜神经胶质细胞单个离子通道的研究 哺乳动物。离子通道的识别，它们的描述 生理学、药理学和调节，可能有助于提供框架 从生物物理水平分析视网膜神经胶质细胞的病理生理学。

The long-term objective of this research plan is to use the patch clamp technique to study the pathophysiology of retinal glial cells. Knowing more about retinal glial cells is desirable since these cells appear to be involved in a number of vitreoretinal disorders. A specific aim is to use the patch clamp technique to study the physiology, pharmacology and regulation of ionic channels in retinal glial cells from adult rats and from humans undergoing vitrectomies. Preliminary findings indicate that rat retinal glial cells can possess at least two kinds of ionic channels. Experiments also show that it is feasible to detect ionic channels in glial cells obtained from patients. The limited knowledge concerning ionic channels of retinal glial cells may be an important gap in our understanding of the pathophysiology of this class of cells. Ionic channels are likely to play important roles in cellular activities under normal and pathophysiological conditions. At present, there are no patch clamp studies, other than in the Preliminary Studies section of single ionic channels in retinal glial cells from mammals. The identification of ionic channels, a description of their physiology, pharmacology and regulation, may help provide the framework for analyzing the pathophysiology of retinal glial cells at a biophysical level.