GENE REGULATION IN LYMPHOHEMOPOIETIC STROMAL CELLS

骨髓基质细胞提供必要的微环境 用于体内淋巴造血。大量的基质细胞克隆 是从长期的骨髓培养中获得的。这 细胞的异质性群体仍然相对不确定， 尊重他们的血统派生。该项目将重点放在 前脂肪细胞基质细胞的组织特异性基因调控 利用细胞和分子生物学相结合的克隆 接近了。单抗与消减杂交 文库筛选将用于鉴定基质细胞特异性 或丰富的基因产品。DNA结合蛋白及其转染法 化验将用于确定反式和顺式作用元件。 调节这些基因。对基质细胞基因的进一步认识 监管，除了增加我们对正常的知识 生理学方面，将对骨髓移植产生影响 恶性肿瘤和遗传性遗传疾病的治疗模式 精神错乱。

Bone marrow stromal cells provide the microenvironment necessary for lympho-hematopoiesis in vivo. A number of stromal cell clones have been derived from long term bone marrow culture. This heterogenous population of cells remains relatively undefined with respect to their lineage derivation. The project will focus on tissue specific gene regulation in pre-adipocyte stromal cell clones using a combination of cell and molecular biological approaches. Monoclonal antibodies and subtractive hybridization library screening will be used to identify stromal cell specific or abundant gene products. DNA binding protein and transfection assays will serve to determine the trans and cis acting elements regulating these genes. Further understanding of stromal cell gene regulation, in addition to increasing our knowledge of normal physiology, will have impact on bone marrow transplantation as a therapeutic modality for malignancy and inherited genetic disorders.