Automation of 3D cell model assembly by additive printing

该项目将通过增加平台组件的打印来测试自动制造先进的基于细胞的分析平台的可行性。分析平台应为3D细胞培养，以增强驻留细胞的功能。添加剂印刷应在与中等吞吐量分析兼容的多孔培养板内的3D结构中一致和高效地沉积生物兼容材料。设计用于模拟组织结构的3D打印结构将填充干细胞来源的群体，其组织特定功能被选择为与打印的结构相匹配；应研究将细胞直接打印到这些结构中的可行性。出于演示目的，应使用来自iPS前体细胞并以肝脏相关3D模式沉积的肝细胞来评估自动添加打印的潜力，并将模型功能与竞争系统和基于药物代谢酶水平的动物模型进行比较。其结果将是将添加剂打印应用于药物发现和其他工业过程中使用的基于3D细胞的分析组装的潜力的工业化前演示。这一演示将为进一步的研究和开发创造一个跳板，旨在将先进的基于电池的模型组装自动化，以达到工业质量标准。

This project shall test the feasibility of automating the manufacture of advanced cell-based analysis platforms by additiveprinting of platform components. The analytical platforms shall be 3D cell cultures which enhance the function of residentcells. Additive printing shall deposit biocompatible materials consistently and efficiently in 3D architectures within multiwellculture plates compatible with medium-throughput analysis. 3D-printed structures designed to mimic tissue architectureshall be populated with stem cell-derived populations with tissue-specific functionality selected to match the printedarchitecture; the feasibility of printing cells directly into these structures shall be studied. For demonstration purposes, thepotential of automated additive printing shall be evaluated using hepatocytes derived from iPS progenitor cells anddeposited in liver-relevant 3D patterns, with model functionality being compared against competing systems and animalmodels based on drug-metabolising enzyme levels. The outcome shall be a pre-industrial demonstration of the potential toapply additive printing in the assembly of 3D cell-based assays used in drug discovery and other industrial processes. Thisdemonstration will create a springboard for further research and development aimed at automation of advanced cell-basedmodel assembly to industrial quality standards.