GROWTH CONE GUIDANCE; INTERACTIONS AND SURFACE MOLECULES

本提案的目标是确定（1）什么样的蜂窝 相互作用对于开拓 蝗虫胚胎中枢神经的轴突通路 系统，（2）后来的神经元如何在其细胞特异性 选择在这个支架的轴突通路，和（3）什么 分子参与引导生长锥在其初始和 后来的选择。 蝗虫胚胎神经 系统将被用来回答这些问题。 该模型系统 由于体内的原因， 它提供的蜂窝分辨率和可访问性。 单个识别的 在已识别的细胞环境中的生长锥可以 在蚱蜢胚胎中观察和操作。 特异性神经元-上皮、神经元-神经胶质和神经元-神经元 相互作用将在光和EM水平上表征，然后 将进行实验操作以确定 每种相互作用在生长锥导向和途径中的作用 选择 单克隆抗体将产生对这同样 一组上皮细胞、神经胶质细胞和神经元细胞， 生长锥所需的特定相互作用的相关性 指导

The goals of this proposal are to determine (1) what cellular interactions are essential for pioneering the initial scaffold of axon pathways in the embryonic grasshopper central nervous system, (2) how later neurons are guided in their cell specific choices among this scaffold of axon pathways, and (3) what molecules are involved in guiding growth cones in their initial and later pathway choices. The grasshopper embryonic nervous system will be used to answer these questions. This model system is uniquely suited for these experiments because of the in vivo cellular resolution and accessibility it offers. Single identified growth cones in an identified cellular environment can be observed and manipulated in the grasshopper embryo. Specific neuron-epithelial, neuron-glial, and neuron-neuron interactions will be characterized at light and EM level and then experimental manipulations will be performed to determine the role of each interaction in growth cone guidance and pathway choice. Monoclonal antibodies will generated against this same set of epithelial, glial, and neuronal cells to find molecular correlates of the specific interactions required for growth cone guidance.