CONTROL OF VIRAL RNA SYNTHESIS IN HERPESVIRUS INFECTION

我们将继续研究结构/功能特征 单纯疱疹病毒1型基因的重要性，在其控制 在生产性感染期间表达。 我们将使用记者 能被反式作用病毒产物激活的质粒 因为它们在操作特定的 参与调控的序列元件。 这些方法将 用于精确鉴定选定病毒中的序列元件 对病毒基因的反式激活有反应的前导序列 产品，并研究模板复制对 不同时间类基因的表达。 序列 在病毒特异性激活中被确定为重要的元素， 转录抑制将被用作探针来研究 病毒控制序列之间相互作用的性质， 在病毒复制过程中表达的特异性调节蛋白。 选择的序列将被修改并重新引入到 病毒本身，因此标记基因表达的改变 在生产性感染可以检查。 我们有 表征了在潜伏期内表达为mRNA的单个基因， 感染，现在将表征抗ICPO基因， 它的序列，它的表达控制，和蛋白质产物 这些信息将被用来操纵 抗ICO的表达，以研究 这种转录本在神经元中的排他性表达以及它的任何作用， 可能会导致感染。

We will continue to investigate the structural/functional features of herpes simplex virus type 1 genes important in their controlled expression during productive infection. We will use reporter plasmids capable of being activated by trans-acting viral product because of their convenience in the manipulation of specific sequence elements involved in regulation. Such methods will be used to precisely identify sequence elements in selected viral leaders that are responsive to trans-activation by viral gene products and to examine the role of template replication on the expression of genes of different temporal classes. Sequence elements identified as important in virus-specific activation and repression of transcription will be utilized as probes to investigate the nature of the interaction between viral control sequences and specific regulatory proteins expressed during viral replication. Selected sequences will be modified and reintroduced into the virus itself so that alterations in expression of marker genes during productive infection can be examined. We have characterized a single gene expressed as mRNA during latent infection and will now characterize the anti-ICPO gene in terms of its sequence, control of its expression, and the protein products encoded by it. Such information will be used to manipulate the expression of anti-ICO to investigate the molecular basis for the exclusive expression of this transcript in neurons and any role it may have in productive infection.