CONTROL OF VIRAL RNA SYNTHESIS IN HERPESVIRUS INFECTION

我们将继续调查结构/功能特征 单纯疱疹病毒1型基因在其控制中的重要作用 在生殖性感染期间的表达。我们将使用记者 能够被反式作用病毒产物激活的质粒 因为它们在操纵特定对象时很方便 对参与调控的元素进行排序。这些方法将是 用于精确识别选定病毒中的序列元素 对病毒基因反式激活有反应的领导者 产品，并研究模板复制在 不同时间类基因的表达。数列 被确认为在病毒特异性激活和 转录抑制将被用作探针来研究 病毒控制序列与病毒之间相互作用的性质 在病毒复制过程中表达的特定调节蛋白。 选定的序列将被修改并重新引入到 病毒本身使标记基因的表达发生变化 在生产过程中可以检查感染情况。我们有 描述了单个基因在潜伏期以mRNA的形式表达 感染，现在将以下列方式表征抗ICPO基因 ITS的序列、表达的控制以及蛋白质产品 由它编码。这些信息将被用来操纵 表达抗ICO抗体以探讨其发病的分子基础 该转录本在神经元中的排他性表达及其作用 可能在生殖性感染中有。

We will continue to investigate the structural/functional features of herpes simplex virus type 1 genes important in their controlled expression during productive infection. We will use reporter plasmids capable of being activated by trans-acting viral product because of their convenience in the manipulation of specific sequence elements involved in regulation. Such methods will be used to precisely identify sequence elements in selected viral leaders that are responsive to trans-activation by viral gene products and to examine the role of template replication on the expression of genes of different temporal classes. Sequence elements identified as important in virus-specific activation and repression of transcription will be utilized as probes to investigate the nature of the interaction between viral control sequences and specific regulatory proteins expressed during viral replication. Selected sequences will be modified and reintroduced into the virus itself so that alterations in expression of marker genes during productive infection can be examined. We have characterized a single gene expressed as mRNA during latent infection and will now characterize the anti-ICPO gene in terms of its sequence, control of its expression, and the protein products encoded by it. Such information will be used to manipulate the expression of anti-ICO to investigate the molecular basis for the exclusive expression of this transcript in neurons and any role it may have in productive infection.