AREA DETECTOR FOR PROTEIN CRYTALLOGRAPHY

我们已经非常成功地将多线计数器面积探测器用于 蛋白质晶体学数据收集在我们的实验室， 加州大学圣地亚哥分校（作为NIH研究资源资助）。 我们已经多次证明这些探测器可以提高速度 从蛋白质晶体中收集X射线数据的效率 与传统的薄膜或单晶衍射相比， 方法. 由此产生的数据也始终比数据更准确 用传统方法收集。 从我们实验室大量的时间请求来看 探测器系统（是我们每年服务的两倍多）， 一个明确的和强烈的需求，商业版本的这一良好的证明， 探测器技术 我们预计这一需求将在2010年迅速增长， 未来几年，由于蛋白质晶体学工作变得非常重要， 不仅用于分子生物学的基础研究， 生物医学领域，如合理的药物设计和蛋白质工程。 建议的工作将集中在使我们现在的成功 通过简化和加固，探测器设计在商业上更实用 了 因此，它将更容易建立在生产数量和简单 操作，所以它可以被那些不需要了解它的人使用。 技术细节。

We have very successfully adapted the multiwire counter area detector to protein crystallographic data collection in our laboratory at the University of California, San Diego (funded as an NIH Research Resource). We have demonstrated many times that these detectors can increase the speed and efficiency at X-ray data collection from protein crystals by a factor of 100 or more compared to traditional film or single-crystal diffraction methods. The resulting data are also consistently more accurate than data collected by traditional methods. Judging from the large number of requests for time on our laboratory detector system (more than double what we can service each year), there is a clear and intense demand for a commercial versiion of this well-proven detector technology. And we expect this demand to grow rapidly in the coming years, since protein crystallographic work has become very important not only for basic research in molecular biology but also in many biomedical areas such as rational drug design and protein engineering. The proposed work will be centered on making our present successful detector design more commercially practical by simplifying and ruggedizing it. It will thus be easier to build in production quantities and simpler to operate, so it can be used by people who do not need to understand its technical details.