X-Ray Detectors for Synchrotron-Based Structural Biology

用于基于同步加速器的结构生物学的 X 射线探测器

• 批准号: 7494218
• 负责人: RONALD C HAMLIN
• 金额: $ 134.2万
• 依托单位: AREA DETECTOR SYSTEMS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7494218
• 关键词: Architecture; Area; Authorization documentation; Bite; California; Charge; Cities; Collaborations; Count; Data; Data Collection; Data Quality; Data Sources; Development; Disclosure; Electrical Engineering; Electronics; Engineering; Face; Human Resources; Image; Instruction; International; Methods; Names; Numbers; Object Attachment; Phase; Physics; Pliability; Process; Proteins; Radiation; Relative (related person); Research; Research Project Grants; Roentgen Rays; Role; Rotation; Signal Transduction; Slice; Software Engineering; Source; Speed; Staging; Standards of Weights and Measures; Structure; Synchrotrons; Testing; Time; Universities; analog; base; design; desire; detector; digital; engineering design; experience; improved; programs; prototype; structural biology; success; voltage

Development is proposed of an x-ray Pixel Array Detector (PAD) consisting approximately of 2,000x2,000 pixels to enhance macromolecular crystallographic data collection at synchrotron sources A PAD consists of an x-ray sensing integrated circuit layer bonded to a pixel electronics layer such that each pixel has its own processing electronics PADs promise to reduce data collection time by up to a factor of 40 relative to existing detectors while improving data quality They will have specific capability to 1 Collect a set of monochromatic data (90 ¿ rotation) in 18 seconds using the standard oscillation method (0 5 ¿ per frame, with exposure time of 100 ms per frame) 2 Collect a set of fine-slice data in 90 seconds with a rotation of 0 05 ¿ per frame and with an exposure of 50 ms per frame 3 Collect frames at microsecond exposure time for time-resolved protein Laue diffraction Research during Phase I of this proposal was successful in identifying a mixed-mode analog/digital pixel architecture best suited for the crystallographic application, as well as demonstrating ways of resolving radiation damage concerns The input signal is analog integrated onto a capacitor, C_nt,by a charge to voltage converter in the charge integrator stage using a structure very similar to the one in the existing APAD designs The voltage on the capacitor is constantly compared to an external reference voltage, Vref, via a voltage comparator in the threshold detector stage When the integrated voltage level reaches the reference voltage, a bit is generated and added into an in-pixel digital counter The threshold detector then also resets the integration capacitor in the charge integrator stage Thus, each analog cycle of filling C_ntresults in an additional digital "count" in the high- order word stored in the in-pixel digital counter At the end of the exposure frame, the voltage level remaining on Gin t is digitized, if desired, by an analog-to-digital converter (ADC) at the end of each row of pixels This digitized value is thus the low-order digital word of the exposure Phase II will involve finalization of the specific pixel layout, fabrication of suitably large-area integrated circuits, assembly and application testing of a full-scale PAD PERFORMANCESITE(S) (organization,city, state) 1 0 ADSC, 12550 Stowe Drive, Poway, CA, 92064 2 0 Cornell University, Physics Dept, Clark Hall, Ithaca, NY 14853 3 0 UCSD, 9500 Gilman Drive, La Jolla, CA, 92093 KEY PERSONNEL See instructions Use continuationpagesas needed to providethe requiredinformation in theformat shownbelow Startwith Principal InvestigatorListall other key personnelinalphabeticalorder,lastnamefirst Name Organization Role on Project Hamlin, Ronald, Ph D ADSC PI Allin, Matt ADSC Technician Angello, Susan, BS ADSC Process Development Electrical Engineer (TBD) BS ADSC Electronic Development Hontz, Tom MBA, MS ADSC Program Director/Pck Engr Nielsen, Chris, MA ADSC Software Engineer Vernon, Wayne, Ph D ADSC Test Engineer Gruner, Sol, Ph D Cornell PAD Development Tate, Mark, Ph D Cornell Design Engineering Nguyen-huu, Xuong, Ph D UCSD PAD Development Disclosure Permission Statement Applicableto SBIPJSTTROnly. Seeinstructions [] Yes [] No PHS 398 (Rev 05/01) face page 2 Form ApprovedThrough 05/2004 OMB No 0925-0001 RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page ..................................... 1 2 Description,

提出了一种由大约2，000 × 2，000个像素组成的X射线像素阵列探测器（PAD）的开发， 增强同步加速器源上的大分子晶体学数据收集PAD由X射线传感器组成， 结合到像素电子器件层的集成电路层，使得每个像素具有其自己的处理电子器件PAD 与现有探测器相比，有望将数据收集时间缩短40倍，同时提高数据质量 他们将有特定的能力， 1使用标准振荡方法（0 5 <$per）在18秒内收集一组单色数据（90 <$旋转） 帧，曝光时间为每帧100 ms） 2在90秒内收集一组精细切片数据，每帧旋转0 05 <$，曝光50 ms 每帧 3以微秒曝光时间收集帧，用于时间分辨蛋白质劳厄衍射 该提案第一阶段的研究成功地确定了混合模式模拟/数字像素 最适合晶体学应用的架构，以及演示解决辐射的方法 输入信号通过电荷-电压转换器模拟积分到电容器C_nt上， 电荷积分器级使用与现有APAD设计中的结构非常相似的结构。 电容器上的电压恒定地与外部参考电压进行比较， 阈值检测器级当积分电压电平达到参考电压时，产生一个位， 然后，阈值检测器还重置像素中的积分电容器。 因此，填充C_n的每个模拟周期导致高电平中的附加数字“计数”。 在曝光帧结束时，保持开启的电压电平为 如果需要的话，Gin t由每行像素末端的模数转换器（ADC）数字化。 因此，值是曝光的低阶数字字。阶段II将涉及特定像素的最终化 布局、适当大面积集成电路的制造、全尺寸PAD的组装和应用测试 地点（组织、城市、州） 1 0 ADSC，12550斯托驱动器，波威，CA，92064 2 0康奈尔大学，物理系，克拉克大厅，伊萨卡，纽约14853 3 0 UCSD，9500 Gilman Drive，拉霍亚，CA，92093 关键人员参见说明根据需要使用续页，以下面所示的格式提供所需信息 从首席调查员开始列出其他关键人员按姓名顺序，姓氏在前 名称组织在项目中的角色 Hamlin，罗纳德，博士ADSC PI Allin，Matt ADSC技术员 Angello，Susan，BS ADSC工艺开发 电气工程师（TBD）BS ADSC电子开发 Hontz，Tom MBA，MS ADSC项目总监/包装工程师 Nielsen，Chris，MA ADSC软件工程师 弗农，韦恩，博士ADSC测试工程师 Gruner，Sol，康奈尔PAD开发博士 Tate，Mark，康奈尔大学设计工程博士 Nguyen-huu，Xuong，博士UCSD PAD开发 披露许可声明仅适用于SBIPJSTTR。参见说明[]是[]否 PHS 398（修订版05/01）主页 2 表格批准日期至2004年5月OMB编号0925-0001 研究资助 目录 页码 首页.... 1 2 产品描述：