BIOCHEMICAL CONTROL OF MICROCARRIER PROCESSES

微载体系统显示出大规模应用的巨大潜力 细胞的生长及其代谢产物的产生。 我们的长期目标是开发一种改进的微载体 生长苛刻的、依赖于锚定的细胞。 的目标 这项研究是为了了解生物化学基础， 在几个细胞上观察到的细胞生长和行为的差异 市售微载体和我们的Bioglas 微载体珠。 具体目标是确定是否存在 内源性蛋白水解酶的水平和 特定的花生四烯酸代谢产物的水平， 各种微载体基质上的细胞有助于 细胞在这些基质上生长和行为的差异。 的 第一阶段的目标将是比较增长， MRC-5细胞在五种微载体（CytodexI， Cytodex III、Ventregel、Bioglas和Bioglas） 条件和条件下，其中的功能， 内源性蛋白酶被抑制。 为了比较细胞生长， 在正常条件下， 生长条件和在其中合成 花生四烯酸的环氧合酶和/或脂氧合酶产物 被调制。 这些结果将有助于开发一种改进的生物玻璃 微载体和更高效的微载体方案。

Microcarrier systems show great potential for the large-scale growth of cells and the production of their metabolic products. Our long-term objective is to develop an improved microcarrier for growing fastidious, anchorage-dependent cells. The goal of this research is to understand the biochemical basis for the differences in cell growth and behavior observed on the several commercially available microcarriers and our Bioglas microcarrier beads. The specific objectives are to determine if the differences in the levels of endogenous proteolytic enzymes and the differences in the levels of specific arachidonic acid metabolites produced by cells on the various microcarrier substrates contributes to differences in cell growth and behavior on these substrates. The Phase I objective will be to compare growth, adhesiveness and morphology of MRC-5 cells on five microcarriers (Cytodex I, Cytodex III, Ventregel, Biosilon and Bioglas) under normal growth conditions and under conditions in which the functioning of endogenous proteases is suppressed. And, to compare cell growth, adhesiveness and morphology on the same substrates under normal growth conditions and under conditions in which the synthesis of cyclooxygenase and/or lipoxygenase products of arachidonic acid is modulated. These results will aid in developing an improved Bioglas microcarrier and more productive microcarrier protocols.