SYNTHESIS OF NEW FLUOROPHORES FOR CYTOSOLIC GLUTATHIONE

我们提出了一种新的荧光团的合成细胞内 谷胱甘肽测定 的反应产物 具有硫醇的荧光团将具有更长的波长吸收， 发射和更高的量子产率比最好的胞质硫醇 荧光团目前用于通过流式细胞术进行细胞分选。 这种染料的优点是可以在细胞膜上 由于酯化的酚和羧基， 随后将被胞质酯酶脱酯化，因此 将所述指示剂捕获在所述单元内。 因此，细胞负载 细胞外染料浓度应远低于 目前可能。 我们还建议研究吸收和发射性质， 谷胱甘肽和巯基的结合和未结合状态， 含有蛋白质。

We propose the synthesis of a new fluorophore for intracellular glutathione determination. The reaction product of the fluorophore with thiols will have longer wavelength absorption and emission and higher quantum yield than the best cytosolic thiol fluorophore currently in use for cell sorting by flow cytometry. The dye will have the advantage of being cell membrane permeable owing to esterified phenol and carboxyl groups which will subsequently be de-esterified by cytosolic esterases, thus trapping the indicator within the cell. Therefore, cell loading should occur at much lower extracellular dye concentrations than currently possible. We also propose to study the absorption and emission properties in both bound and unbound states with glutathione and thiol- containing proteins.