PRODUCTION OF REFERENCE REAGENTS FOR SAIDS AND STLV-III

在非人类灵长类动物中两种不同的病毒诱导的感染，导致 相当高的发病率和死亡率，与人类临床相关 最近发现了艾滋病。第一个，被称为SAID，是由 由多种密切相关的D型病毒引起。第二种是由一种 在形态、生物学和血清学上有关联的LentaVirus 人类HTLV-III和HTLV-IV，命名为STLV-III。这些感染 之所以重要，是因为它们：(A)感染许多非人类灵长类动物，并可能 对这些物种的实验有重大影响，以及(B)可用作 潜在的研究生物学、发病机制和潜力的模型 艾滋病类疾病的治疗。 微生物协会公司(MAI)建议培养出 Raji细胞中的SAID(SRV-1；SRV-2)病毒和HUT-78中的STLV-III病毒 细胞。标准化试剂(即病毒感染细胞，两者均为同源 感染猴抗血清和异种兔抗血清 用整个病毒分离株免疫)将被表征。这些 然后将使用试剂来开发快速鉴定的分析方法 暴露在病毒下的动物。

Two distinct virally induced infections in non-human primates which cause substantial morbidity and mortality and with clinical relevance to human AIDS have been identified recently. The first, designated SAIDS, is caused by a variety of closely related Type D viruses. The second is induced by a lentavirus which is morphologically, biologically and serologically related to human HTLV-III and HTLV-IV and is designated STLV-III. These infections are of importance because they: (a) infect many non-human primates and may have major effects on experimentation in these species and (b) can serve as potential models for examining the biology, pathogenesis and potential therapy of AIDS-like diseases. Microbiological Associates, Inc. (MAI) proposes to grow isolates of the SAIDS (SRV-1; SRV-2) viruses in Raji cells and the STLV-III virus in HUT-78 cells. Standardized reagents (i.e. virally infected cells, both homologous antisera from infected monkeys and heterologous antisera from rabbits immunized with the whole virus isolates) would be characterized. These reagents would then be used to develop assays for the rapid identification of animals exposed to the virus.