VASOACTIVE HORMONES AND BLOOD PRESSURE REGULATION

这是一项继续描述 肾脏的管理和职能职责领域 激肽释放酶-激动素和前列腺素系统，相互作用的激素 有助于调节肾功能和 血压。我们建议在以下方面进行试验。 领域1：机制和生理方面的研究 激肽对肾脏的调节作用及其意义 功能。实验将在灌流的肾脏和 分离的肾小球和肾小管(A)以表征肾脏 激肽在受体参与和相互关系中的作用 对cGMF和前列腺素合成(B)的影响进行研究 纯化激肽原对肾功能和肾内生成的影响 金宁。前列腺素和cGMP；和(C)评估肾脏 激动素受体拮抗剂治疗的功能反应 有过量的加压素或蛋白质摄入过多，哪些情况下 已知会增加肾脏激动素。 区域2：研究激肽对血液的贡献 肾动脉结扎对肾动脉的降压作用 高血压。将进行实验(A)以调查 肾动脉的降压反应与血压的关系 解夹及血浆和肾脏分泌物的变化 以及(B)评估激动素受体拮抗剂对 松解时的低血压反应。 领域3：激动素之间关系的调查， 前列腺素合成与甘油三酯在肾脏中的代谢 骨髓细胞。实验将在培养的兔身上进行 肾髓质间质细胞(A) RMIC的甘油三酯脂肪酶及其激动素的调节作用 CAMP介导的肾1激素和渗透压；和(B)检查 激动素和其他激素对甘油三酯的刺激作用 脂酶活性对重延髓合成二十烷类化合物的影响 间质细胞。

This is a proposal to continue characterizing the mechanisms of regulation and areas of functional responsibility of the renal kallikrein-kinin and prostaglandin systems, interacting hormonal systems which contribute to the regulation of renal function and blood pressure. We propose experimentation in the following areas. Area 1: Investigation of the Mechanisms and Physiological Significance of the Regulatory Influence of Kinins on Renal Function. Experiments will be conducted in perfused kidneys and isolated glomeruli and tubules (a) to characterize the renal actions of kinins in terms of receptors involved and relationship to cGMF and prostaglandin synthesis (b) to investigate the effects of purified kininogen on renal function and intrarenal generation of kinins. Prostaglandins and cGMP; and (c) to assess the renal functional response to treatment with kinin receptor antagonists with excess vasopressin or high protein intake, which conditions are known to increase renal kinins. Area 2: Investigation of the Contribution of Kinins to the Blood Pressure Lowering Effect of Renal Artery Unclipping in Renal Hypertension. Experiments will be conducted (a) to investigate relationships between the hypotensive response to renal artery unclipping and changes in plasma levels and renal secretion of Kinins, and (b) assess the effect of Kinin receptor antagonists on the hypotensive response to unclipping. Area 3: Investigation of the Relationship Between Kinins, Prostaglandin Synthesis and Trizlvceride Metabolism in Renal Medullary Cells. Experiments will be conducted in cultured rabbit renomedullary interstitial cells (a) to characterize the triglyceride lipase of RMIC and study its regulation by kinins, cAMP-mediated renal 1 hormones and osmolality; and (b) to examine the effects of kinins and other hormone that stimulate triglyceride lipase activity on eicosanoid synthesis by renomedullary interstitial cells.