CONSTRUCTION OF CHICKEN X MOUSE HYBRIDOMAS

描述：（改编自申请人的摘要） 鸡X小鼠杂交瘤细胞系分泌鸡单克隆抗体，以选择 提出了抗原。 在这些构造中的主要优点是 杂交瘤是鸡和哺乳动物之间的遗传差异， 增加了产生抗体的机会， 哺乳动物蛋白质如肽激素和生长因子的区域。 由于这种遗传差异，鸡单克隆抗体可能是 制备成在小鼠中具有弱免疫原性或非免疫原性的表位， 大鼠 种间杂交瘤已经通过融合免疫 来自许多哺乳动物物种的淋巴细胞与小鼠骨髓瘤细胞。 这些杂交瘤主要用于该物种特有的研究。 然而，鸡单克隆抗体技术可能变得与 目前的小鼠和大鼠单克隆抗体技术。 抗体可以找到 作为研究试剂、治疗剂的商业应用，以及 诊断试剂盒。 第一阶段申请的主要目的是 研究了制备的可行性和最佳条件， 分泌鸡单克隆抗体的鸡×小鼠杂交瘤 抗原的 将通过稳定性、生长和细胞周期监测杂交瘤。 免疫球蛋白生产。 因为已经证明， 用小鼠骨髓瘤细胞系制备的种间杂交瘤， 一般不稳定，次要目标将包括检查 选择性鸡X小鼠异骨髓瘤作为融合伴侣， 当与免疫鸡淋巴细胞融合时支持稳定的杂交瘤。

DESCRIPTION: (Adapted from the applicant's abstract) The construction of chicken X mouse hybridoma cell lines secreting chicken MAbs to selected antigens is proposed. A primary advantage in the construction of these hybridomas is the genetic differences between chickens and mammals which increases the chance of producing antibodies against highly conserved regions of mammalian proteins such as peptide hormones and growth factors. Because of this genetic difference, chicken MAbs could potentially be prepared to epitopes that are either weakly or non-immunogenic in mice or rats. Interspecies hybridomas have been prepared by fusing immune lymphocytes from a number of mammalian species with mouse myeloma cells. These hybridomas are primarily useful for studies unique to that species. However, chicken MAbs technology could potentially become as important as current mouse and rat MAb technology. The antibodies could find commercial application as research reagents, therapeutics, and in diagnostic kits. The major objective of the Phase I application is to examine the feasibility and optimal conditions for the preparation of chicken X mouse hybridomas secreting chicken MAbs to a selected model antigen. The hybridomas will be monitored by stability, growth and immunoglobulin production. Since it has been demonstrated that interspecies hybridomas prepared with mouse myeloma cell lines are generally unstable, a secondary objective will include the examination of selective chicken X mouse heteromyelomas as fusion partners that will support stable hybridomas when fused with immune chicken lymphocytes.