EXPRESSION OF SINGLE-CHAIN FV IN NEUROSPORA CRASSA

丝状真菌，由于它们能够分泌高水平的 蛋白质，是有吸引力的生物体的表达和分泌， 具有商业和医学价值的异源蛋白质。 分泌物是关键 在他们的吸引力的元素，分泌提供了机会， 复杂的蛋白质折叠和处理适当和分泌将 有利于净化。 正确的折叠和加工，简化 纯化和廉价的培养转化为更便宜的 生产 我们已经证明了一种分泌和加工， 来自脉孢菌属的具有酶活性的哺乳动物蛋白（前凝乳酶原） crassa，我们建议测试表达一个单一的可行性 链抗体片段。 利用脉孢菌表达 新型单链抗体。

The filamentous fungi, owing to their ability to secrete high levels of proteins, are attractive organisms for the expression and secretion of heterologous proteins of commercial and medical value. Secretion is a key element in their attraction, for secretion provides the opportunity for complex proteins to fold and be processed properly and secretion will facilitate purification. Proper folding and processing, simplified purification, and inexpensive culturing translate into less expensive production. We have demonstrated secretion and processing of an enzymatically active mammalian protein (preprochymosin) from Neurospora crassa, and we propose to test the feasibility of expressing a single chain antibody fragment in Neurospora. The use of Neurospora to express single chain antibodies in novel.