IMAGE-PRESERVING TUNABLE FILTER FOR MICROSCOPY

一种用于细胞显微镜的液晶保像可调谐滤波器 本文提出 双折射液晶技术的最新进展 应该可以构建一种廉价的大孔径可调 在几毫秒内从350 nm到3000 nm范围内无移动的过滤器 部分和6 nm的带通。 有大量的显微镜光学探测技术用于 研究动力学、化学过程和膜运输 活细胞的机制。 这些技术的功效 在很大程度上取决于检测激发、发射或 通过监测窄带宽的光的吸收光谱。 随着更多 探测技术被开发并且系统变得更加复杂， 自动化、多功能性、速度和便利性将越来越 重要. 一个警察工作队将有助于在许多这些领域促进这种艺术。 第一阶段的研究将包括建造一个原型， 液晶技术，并进行可行性研究， 获得注射了钙离子的细胞的钙离子分布的图像 INDO-1。 图像分析并与标准品拍摄的图像进行比较 干涉滤光片将显示当前液体技术的各个方面 需要在第二阶段发展。 它也将表明实际的 设计一个通用的、方便的显微镜附件的考虑因素。

A liquid crystal image-preserving tunable filter (IPTF) for cell microscopy is proposed. Recent developments in birefringent liquid crystal technology should make it possible to construct an inexpensive, large-aperture tunable filter that ranges from 350 to 3000 nm in a few milliseconds with no moving parts and a bandpass of 6 nm. There are a great number of microscopy optical probing techniques used to study the dynamics, the chemical processes and the membrane transport mechanisms of the living cell. The efficacy of many of these techniques depends strongly on ability to detect shifts in the excitation, emission or absorption spectra by monitoring narrow bandwidths of light. As more probing techniques are developed and systems become more complicated, automation, versatility, speed, and convenience will be increasingly important. An IPTF would help to advance the art in many of these areas. Phase I research will include construction of a prototype with existing liquid crystal technology and performing a feasibility study involving the acquisition of images of calcium ion distribution of a cell injected with INDO-1. Image analysis and comparison with images taken with standard interference filters will indicate aspects of current liquid technology that need developing in Phase II. It will also indicate practical considerations for design of a versatile, convenient microscope accessory.