DESIGN AND EVALUATION OF A TRACHEAL PROSTHESIS

第一阶段的研究表明，一种复合气管假体 由坚硬的外层和内部可生物降解的层组成 能成功替代犬颈部气管7.5厘米 无感染、无排斥或无排泄。假体 通过生长和迁移允许管腔内上皮化 通过自由操作的口腔黏膜移植。 第二阶段的研究将继续评估一种刚性化合物 气管假体替代4-6 cm颈椎 气管。第二项研究将评估坚硬的复合气管 加宽饲养羔羊气管和声门下腔的补片 走向成熟。第三项研究将开发和评估灵活的 复合气管假体置换长达10厘米的胸腔 气管。 提高假体上皮化率的方法 使用掺入生长因子、细胞外基质的管腔 内层膜基质，游离手术颊粘膜 移植物，以及术前体外组织培养移植物 已评估。

The Phase I study demonstrated that a compound tracheal prosthesis consisting of a rigid outer layer and an inner biodegradable layer can successfully replace 7.5 cm of cervical trachea in the dog without infection, rejection, or extrusion. The prothesis permitted intraluminal epithelization by growth and migration and by free operative buccal mucosal grafts. The Phase II study will continue to evaluate a rigid compound tracheal prosthesis as a replacement for 4-6 cm of cervical trachea. A second study will evaluate a rigid compound tracheal patch to widen the tracheal and sub-glottic lumen in lambs raised to maturity. A third study will develop and evaluate a flexible compound tracheal prosthesis to replace up to 10 cm of thoracic trachea. Methods to increase the rate of epithelization of the prosthetic lumen using incorporation of growth factors, extracellular basement membrane matrix on the inner layer, free operative buccal mucosal grafts, and in-vitro preoperative tissue culture grafting will be evaluated.