CHOLECYSTOKININ ANALOGUES

本文报道了19个八肽胆囊收缩素类似物的合成。 这些肽的制备将涉及固相技术， 在一种新的逆流色谱仪上纯化， 卧式直通螺旋管行星离心机。 这些约束力 缩胆囊素中枢神经系统和胰腺受体肽 将被确定。 此外，还将筛选肽， 在动物行为模式和豚鼠中的激动剂活性或拮抗作用 猪胰腺腺泡细胞分泌。 因此，潜在的中心， 行为和生理活性肽，适用于研究 将确定胆囊收缩素功能。 这些肽是由 可用于食物相关行为研究和临床前研究 在神经和胃肠道疾病领域。 方法 为了制备大量的最具活性的肽， 开发 这些措施包括化学合成程序的修改 并使用定制的螺旋式行星离心机 为此目的。

The syntheses of 19 cholecystokinin-octapeptide analogues are proposed. The preparation of these peptides will involve solid-phase techniques and purification on a new instrument for counter-current chromatography, the horizontal flow-through coil planet centrifuge. The binding of these peptides to cholecystokinin central nervous system and pancreatic receptors will be determined. Additionally, the peptides will be screened for either agonist activity or antagonism in animal behavior paradigms and in guinea pig pancreatic acinar cell secretion. Thus, potential centrally, behaviorally and physiologically active peptides, suitable for the study of cholecystokinin function will be identified. The peptides are to be made available for research in food-related behaviors and pre-clinical research in areas relating to neurological and gastrointestinal diseases. Methods to prepare large amounts of the most active peptides are proposed to be developed. These include modification of the chemical synthetic procedure and use of a preparative-scale coil planet centrifuge to be custom built for this purpose.