SCREENING TEST FOR CYSTIC FIBROSIS CARRIERS (PHASE II)

囊性纤维化（CF）是影响人类健康的最常见的致死性遗传疾病。 美国和欧洲的白色人口。 这个目标 一个项目是利用重组DNA技术来获得信息 设计CF基因携带者的检测方法。 项目一期 将从大CF的成员建立淋巴母细胞系 家谱，以提供来自家庭成员的DNA来源。 这些DNA将是 用于第2阶段，使用DNA确定每个个体的基因型 检测限制性片段长度多态性的探针。 的 CF基因的染色体定位将通过遗传连锁来确定 这样的DNA标记。 染色体特异性克隆程序将在 用于CF致病基因的克隆和鉴定。 这 关于CF基因产物的信息将被用于设计一个 CF基因携带者的可靠测试，可用于筛选所有成年人 在一般人群中。

Cystic fibrosis (CF) is the most common lethal genetic disease affecting the white population in the United States and Europe. The goal of this project is to use recombinant DNA technology to gain the information necessary to design a test for CF gene carriers. In Phase I of the project lymphoblastoid cell lines will be established from members of large CF pedigrees to provide a source of DNA from family members. This DNA will be used in Phase 2 to determine the genotype of each individual using DNA probes that detect restriction fragment length polymorphisms. The chromosomal location of the CF gene will be determined by genetic linkage to such DNA markers. Chromosome specific cloning procedures will then be applied to cloning and characterizing the gene causing CF. This information concerning the CF gene product will be used to design a reliable test for CF gene carriers that could be used to screen all adults in the general population.