DEVELOPMENT OF AN AUTOMATED SEGMENTED DNA SYNTHESIZER

这项建议涉及开发一种自动化DNA 合成器的数量级更快，更具成本效益 比目前的商业DNA合成器，同时利用标准的DNA 化学. 本研究提案的目标是自动化 用于晶片DNA合成装置的溶剂输送系统， 最终使设备的所有方面自动化。 一旦完成 实现了自动化，Wafer DNA合成装置有望 将每次碱添加的试剂成本从目前的2降低到5 美元（0.2 μ mol规模的常规DNA合成 合成器）到50美分以下。 一个人的表演能力 自动化机器，其更快的生产时间和更低的成本 生产应大大减少相关的成本和时间 测序项目，基因合成，蛋白质工程，DNA 扩增、探针优化和其他基因工程 research. 需要数十万到数百万的 单是人类基因组计划的多核苷酸，我们相信， 将半自动Wafer DNA合成仪发展成为全自动DNA合成仪。 自动化机器将对DNA的发展产生重大影响 相关研究。

This proposal relates to the development of an automated DNA synthesizer that is orders of magnitude faster and more cost effective than current commercial DNA synthesizers while utilizing standard DNA chemistry. The goal of this research proposal is to automate the solvent delivery system for the Wafer DNA synthesis device and to eventually automate all aspects of the device. Once complete automation is achieved, the Wafer DNA synthesis device is expected to reduce the reagent cost per base addition from the current two to five dollars (o.2 mumol scale of synthesis by conventional DNA synthesizers) to under fifty cents. The performance capability of one automated machine with its faster production time and lower cost of production should dramatically reduce the cost and time associated with sequencing projects, gene synthesis, protein engineering, DNA amplification, probe optimization and other genetic engineering research. With the need for hundreds of thousands to millions of polynucleotides for the Human Genome project alone, we believe the development of the semi-automated Wafer DNA synthesizer into a fully automated machine will have a major impact in the progress of DNA related research.