DETECTION OF GERM CELL DAMAGE IN RODENTS

描述（改编自申请人摘要）：灵敏、可重现 需要成本效益高的方法来评估 化学物质，以诱导男性，尤其是女性生殖细胞的DNA损伤。 单细胞凝胶（SCG）电泳分析是一种敏感的方法， 检测DNA中的单链DNA断裂和碱不稳定位点 单个细胞。 使用由几千个细胞组成的样本， 测定可以提供关于细胞间分布的信息， 小鼠生殖细胞（精母细胞和 圆形精子细胞）。 拟议研究的目的 （一）进一步明确本办法的敏感性和适用性; 检测男性不同类型生殖细胞DNA损伤的技术 暴露于诱变剂和非诱变剂的小鼠。 血液白细胞用于 提供关于体细胞与生殖细胞损伤水平的信息：（ii） 为了确定该技术是否可用于检测卵巢癌中的DNA损伤， 诱变剂处理的雌性小鼠的细胞：（iii）将数据库扩展到 第二物种（例如，大鼠）。 技术上简单的 男性/女性生殖细胞检测将大大增加生殖细胞研究， 政府机构和私营部门。

DESCRIPTION (Adapted from applicant's abstract): Sensitive, reproducible and cost-effective methods are needed to evaluate the potential of chemicals to induce DNA damage in male and especially, female germ cells. The Single Cell Gel (SCG) electrophoresis assay is a sensitive method for detecting single strand DNA breaks and alkali-labile sites in the DNA of individual cells. Using samples consisting of a few thousand cells, this assay can provide information on the intercellular distribution and persistence of DNA damage induced in mouse germ cells (spermatocytes and round spermatids) by chemical agents. The purpose of the proposed study is: (i) To further characterize the sensitivity and suitability of this technique for detecting different classes of germ cell DNA damage in male mice exposed to mutagens and nonmutagens. Blood leukocytes are used to provide information about somatic versus germ cell levels of damage: (ii) To determine if the technique can be used to detect DNA damage in ovarian cells from mutagen treated female mice: (iii) To extend the data base to a second species (e.g., rat). The availability of a technically simple male/female germ cell assay would greatly increase germ cell research by government agencies and the private sector.