DEVELOPMENT OF AN AUTOMATED SEGMENTED DNA SYNTHESIZER

描述（改编自申请人摘要）：本提案涉及 自动化的同时化学合成大量的高 高质量的寡核苷酸，总通量大于4个碱基/ 使用β-氰乙基亚磷酰胺化学和试剂使用 在传统的自动化DNA合成仪的一小部分。其中一个目标是 第二阶段的研究是制造一个完全自动化的多个DNA 能够同时产生40 - 60个寡核苷酸的合成器， 0.02- 0.2 μ mol规模，降低了从反应器中每次碱添加的试剂成本。 目前的1.00美元至2.00美元（0.2 umol规模的常规DNA合成 合成器）到50美分以下。自动合成器的性能能力 机器的生产时间更快，生产成本更低， 大大减少与研究项目相关的成本和时间 需要大量的寡核苷酸。 除了制造一个完全自动化的系统， 成本负担得起的研究人员，另一个第二阶段的目标是产生一个 例如，用于HGP的高通量半自动化仪器 寡核苷酸文库、STS引物、库和寡核苷酸 阵 第二阶段的研究预计将刺激进一步的技术 HGP的发展。

DESCRIPTION (Adapted from applicant's abstract):This proposal relates to the automated simultaneous chemical synthesis of large numbers of high quality oligonucleotides at a total throughput greater than four bases per minute using beta-cyanoethyl phosphoramidite chemistry, and reagent usage at a fraction of conventional automated DNA synthesizers. One goal of this Phase II research is to fabricate a fully automated, multiple DNA synthesizer capable of producing 40 - 60 oligonucleotides simultaneously at 0.02 - 0.2 umol scale, reducing the reagent cost per base addition from the current $1.00 to $2.00 (0.2 umol scale of synthesis by conventional DNA synthesizers) to under 50 cents.The performance capability of the automated machine with its faster production time and lower cost of production should dramatically reduce the cost and time associated with research projects requiring numerous oligonucleotides. Besides fabricating a fully automated system which could be produced at a cost affordable to researchers, another Phase II objective is to produce a higher throughput, semiautomated instrument for the HGP, for example oligonucleotide libraries, STS primer, repository and oligonucleotide arrays. The Phase II research is expected to stimulate further technology development in the HGP.