CYTOSOLIC CA2+IONOPHORES FOR FLUORESCENCE AND L9F NMR

细胞内钙是目前广泛关注的主题， 触发和调节细胞激活的第二信使。 一系列 可用于19 F NMR的改进的Ca 2+螯合剂， 荧光检测将被合成并评估其 吸收和发射特性以及它们结合 Ca2+。 所提出的Ca 2+指示剂将具有改善的光学性能， 与目前使用的quin 2、fura-2和indo-1相比， 除了荧光团之外，结构上相似。 此外该 指示剂将被氟取代，用于19 F NMR检测。 螯合剂 可用于跟踪细胞内钙浓度的变化， [Ca2+]i，作为响应于各种扰动的时间的函数 影响。 随着越来越多的人认识到跨膜钙流的变化 细胞内钙水平在许多形式的刺激中都很重要 反应偶联，引入Ca 2+特异性试剂， 与荧光和19 F NMR检测一起使用， 为研究细胞功能提供了更多的机会。 它可以用 用于单细胞成像、流式细胞术、细胞功能研究和NRM 灌注器官的研究。

Intracellular calcium is currently the subject of widespread interest as a second messenger that triggers and regulates cells activation. A series of improved Ca2+ chelating agents which can be used for 19F NMR and fluorescence detection will be synthesized and evaluated for their absorption and emission characteristics as well as their ability to bind Ca2+. The proposed Ca2+ indicators will have improved optical characteristics over currently used quin2, fura-2 and indo-1, which they resemble structurally except for the fluorophore. In addition, the indicator will be fluorine substituted for 19F NMR detection. The chelator can be used to follow changes in intracellular calcium concentration, [Ca2+]i, as a function of time in response to various perturbing influences. With increasing recognition that changes in transmembrane calcium fluxes and intracellular calcium levels are important in many forms of stimulus- response coupling, the introduction of a Ca2+ specific agent that can be used with both fluorometric and 19F NMR detection promises to provide additional opportunities for the study of cell function. It can be used for single cell imaging, flow cytometry, cell function studies and for NRM study of perfused organs.