CHARACTERIZATION OF THE FLUORESCENT PROPERTIES OF ZINC PORPHYRINS

该项目旨在改善目前的光敏化状态 用于肿瘤疾病的检测和光动力治疗。 由于血卟啉、衍生剂和光敏剂的价值都很高 记录下来，这个项目将检查电子和荧光 HPD和Photofrin-II的三种已知成分的作用： 原卟啉、血卟啉和双血卟啉醚/酯。这些 三种预防性药物将通过紫外线/可见光和 激发/发射荧光分光光度法测定无金属和 金属锌的形状。无金属和锌金属卟啉将 在PBS(磷酸盐缓冲盐水；pH-7.2)中进行检查，无细胞和 在含有PTK2细胞的PBS溶液中(大鼠袋鼠上皮肾 单元格)。含有PTK2细胞的各自的卟啉溶液将 也被从染料激光器2受光照射来检查 无金属和金属锌卟啉的光毒性。 本项目的具体目标是测试锌取代的卟啉 针对HpD和Photofrin-II已知成分的络合物。其影响 要考察的内容包括：1)。识别和分配 电子跃迁。2)。荧光寿命的比较 和量子产率，3)。光杀灭的测量和比较 比率。HPD和Photofrin-II组分的比较 金属锌类似物很重要，因为众所周知 金属卟啉的荧光和磷光能力比 他们的非金属同行。如果可以证明锌被取代了 例如，原卟啉表现出比 原卟啉以去金属形式存在，该络合物可用作 癌细胞的定位器。如果这些锌卟啉中的任何一个 它们对细胞表现出优异的敏感度，可用于 光敏剂，作为HPD的前体或与HPD结合使用。

This projects seeks to improve the current state of photosensitization used in the detection and photodynamic therapy of neoplastic disease. Since the value of Hematoporphyrin Derative and Photofrin are well documented, this project will examine the electronic and fluorescent effects of three of the known components of HpD and Photofrin-II: protoporphyrin, hematoporphyrin, and dihematoporphyrin ether/ester. These three prophyrins will be examined by ultraviolet/visible and excitation/emission fluorescence spectrophotometry in the metal free and metallated Zinc forms. The metal free and Zinc metallated porphyrins will be examined in PBS (phosphate buffered saline; ph-7.2) free of cells and in PBS solutions containing PTK2 cells (rat kangaroo epithelial kidney cells). The respective porphyrin solutions containing the PTK2 cells will also be subjected to photoirradiation form a dye laser2 to examine the phototoxicity of the metal-free and metallated Zinc porphyrins. The specific aims of this project are to test Zinc substituted porphyrin complexes against known components of HpD and Photofrin-II. The effects to be examined include the following: 1). identification and assignment of electronic transitions. 2). a comparison of the fluorescence lifetime and quantum yields, 3). measurements and comparisons of the photokill ratios. The comparison of HpD and Photofrin-II components against their metallated Zinc analogues is important because it is well known that metalloporphyrins are better able to fluoresce and phosphoresce than their demetallated counterparts. If it can be shown that Zinc substituted protoporphyrin, for example, exhibits a superior fluorescence than does protoporphyrin in the demetallated form, this complex could be used as a localizer for cancer cells. If any one of these Zinc-porphyrins exhibited superior sensitization of cells, they could be used as photosentizers, either as precursors for or in conjunction with HpD.