CIRCULAR DICHROISM INSTRUMENTATION

要求为圆二色性分光偏振计提供资金。 UNC-CH没有CD仪器。 包括通过观察蛋白质二级结构 棉花对紫外线的影响。 蛋白质和肽将被 研究包括：（1）酵母的天然和变性变体 酿酒酵母iso-1-细胞色素c，（2）含有γ- 在存在和不存在二价金属离子的情况下的羧基谷氨酸 和/或带负电荷的磷脂，（3）核糖体及其 辅因子，（4）凝血调节蛋白和肽 系统中存在和不存在糖胺聚糖，（5）非遗传 合成的具有特殊结构和化学性质的蛋白质 （6）聚肽-二氧化硅结构。 线性二色性将 也可以检查该多肽-二氧化硅结构。 紫外CD光谱将用于分配的绝对配置 过渡金属配合物对合成高纯 分子生物技术。 将检查可见光区的CD 确定手性金属有机化合物的绝对构型 用于对映选择性有机反应的络合物。 可见光中的CD 和近红外区域以及磁性CD将被检查， 确定细胞色素c变体的血红素环境， Co（II）取代胞苷活性中心几何构型的研究 脱氨酶。

Funds are requested for a circular dichroism (CD) spectropolarimeter. There is no CD instrument at UNC-CH. Most of the experiments proposed involve examination of protein secondary structure by observation of Cotton effects in the ultraviolet. The proteins and peptides to be studied include: (1) native and denatured variants of Saccharomyces cerevisiae iso-1-cytochrome c, (2) coagulation proteins containing gamma- carboxy glutamic acid in the presence and absence of divalent metal ions and/or negatively-charged phospolipids, (3) ribosomes and their co-factors, (4) regulatory proteins and peptides of the coagulation system in the presence and absence glycosaminglycans, (5) non-genetic proteins synthesized to have special structural and chemical properties and (6) ultrathin polypeptide-silica structures. Linear dichroism will also be examined for the ultrathin polypeptide-silica structures. Ultraviolet CD spectra will be used to assign absolute configurations of transition metal complexes of importance for synthesis of highly-pure molecules for biotechnology. CD in the visible region will be examined to determine the absolute configuration of chiral organometallic complexes used for enantioselective organic reactions. CD in the visible and near infrared regions as well as magnetic CD will be examined to determine the heme environment of cytochrome c variants and for investigation of the active site geometry of Co(II)-substituted cytidine deaminase.