STORAGE PHOSPHOR IMAGING SYSTEM

一种用于产生、存储和分析图像的系统， 电泳得到的放射性标记DNA、RNA和蛋白质，Southern-， 北方、西方和斑点印迹和薄层色谱图将 后天的 同样的成像系统也将记录x射线衍射 用于确定三维结构的图案 蛋白质和蛋白质与DNA和RNA的复合物。 该系统中的探测器将是光激励荧光屏， 当暴露在β射线和X射线下时会形成潜像。 潜像 通过激光扫描仪转换成数字图像。 光激励 磷光体检测提供了辐射测量的显著增加 灵敏度、定量准确度和动力学范围， 传统的X射线胶片。 荧光屏具有更好的空间分辨率， 可以获得更大的图像，并允许更灵活地收集图像 数据与其他已开发的替代X的技术相比， 射线胶片 图像数据系统由三个主要部分组成。 计算机 受控的激光扫描仪将把潜像转录在荧光体中 屏幕变成数字形式。 数字图像将被传输到 磁盘的多用户文件服务器，这将提供方便的短期 访问和具有成本效益的图像文件的长期存储。 图像将 从服务器传输到分析工作站， 由系统的主要用户提供。 由34名科学家组成的8个研究小组将成为 系统的主要用户。 每个主要用户组都有活跃的或 等待NIH的支持。 所有这些都位于生物系， 布鲁克海文国家实验室。 该系统还将提供给七个 来自生物学研究所的其他研究小组（共25名科学家） 化学系和医学系的科学家， 纽约州立大学的布鲁克海文研究生院和医学院 斯托尼布鲁克的约克和普拉姆岛动物疾病中心。

A system to produce, store and analyze images of the distribution of radiolabeled DNA, RNA and proteins in electrophoretic gets, Southern-, northern-, western-, and dot-blots and thin-layer chromatograms will be acquired. The same imaging system will also record x-ray diffraction patterns that are used to determine the three dimensional structures of proteins and complexes of proteins with DNA and RNA. The detectors in this system will be photostimulable phosphor screens that form a latent image when exposed to beta- and x-rays. The latent image is converted into a digital image by a laser scanner. Photostimulable phosphor detection provides significant increases in radiometric sensitivity, accuracy of quantitation and dynamics range compared to conventional x-ray film. Phosphor screens have better spatial resolution, can acquire larger images, and permit more flexible collection of image data compared to other technologies that have been developed to replace x- ray film. The image data system consists of three major components. A computer controlled laser scanner will transcribe the latent image in a phosphor screen to a digital form. The digital image will be transferred to the disk of a multi-user file server, which will provide convenient short-term access and cost-effective long-term storage of images files. Images will be transferred from the server to analysis workstations that will be provided by the Major Users of the system. Eight research groups, consisting of thirty four scientists, will be the Major Users of the system. Each Major User Group has either active or pending support from NIH. All are located in the Biology Department of Brookhaven National Laboratory. The system will also be available to seven additional research groups (a total of 25 scientists) from the Biology Department and to scientists from the Chemistry and Medical Departments at Brookhaven, the graduate and medical schools of the State University of New York at Stony Brook and the Plum Island Animal Disease Center.