STUDIES ON DNA CONDENSATION AND PROTEIN CONFORMATION

这是一项获得现代多可选光谱偏振仪的建议 为新墨西哥大学化学系工作。这 仪器将具有执行磁环的能力 二向色性，2)荧光检测圆二向色性，3)快速 大量光谱的扫描和平均，4)海量数据存储 数据处理附件和专用软件，5) 低温性能，以及6)动态止流附件 学习。该仪器的三个主要使用者将是卡洛斯博士 Bustaante(化学系)，Mark R.Ondrias博士(化学 David G.Bear博士(细胞生物系)。Dr。 布斯塔曼特建议研究和表征DNA在 通过在体外跟随所谓的Psi型CD异常的出现， 以致密的DNA形式观察到。最近，一种关于这些效应的理论 是在布斯塔曼特博士的实验室里研发出来的。马克·R·昂德里亚斯博士 目前正在研究的暂态和稳态拉曼光谱 多种血红素蛋白。昂德里亚斯博士建议将 利用磁性圆二向色性氧化捕获的光解瞬变 光谱学。大卫·G·贝尔博士建议研究 转录终止因子Rho通过以下方式与特定的信使RNA结合 结合时引起的圆二向色性的变化。每名协管人。 将有权访问的总可用用户时间的25% 乐器。此外，这项提案还包含以下贡献 弗里茨·S·艾伦教授是主用户组之外的用户。Dr。 艾伦计划研究变构调节的分子机制。 肝脏磷酸果糖激酶，通过进行停流研究和 在伴随NAD/NADH转换的CD变化之后。

This is a proposal to obtain a modern multi-optional spectro polarimeter for the Chemisty Department of the University of New Mexico. This instrument will have the capabilities to perform 1) magnetic circular dichroism, 2) fluorescence-detected circular dichroism (FDCD), 3) fast scanning and averaging of large number of spectra, 4) large data storage accessories and specialized software for data processing, 5) low-temperature capabilities, and 6) stopped flow accessory for kinetic studies. The three main users of this instrument will be Dr. Carlos Bustamante (Chemistry Department), Dr. Mark R. Ondrias (Chemistry Department), and Dr. David G. Bear (Cell Biology Department). Dr. Bustamante proposes to study and characterize the condensation of DNA in vitro by following the appearance of the so-called Psi-type CD anomalies, observed in compacted forms of DNA. Recently, a theory of these effects has been developed in Dr. Bustamante's laboratory. Dr. Mark R. Ondrias is currently studying the transient and steady state Raman spectra of multi-heme proteins. Dr. Ondrias proposes to characterize the state of oxidation of trapped photolytic transients via magnetic circular dichroism spectroscopy. Dr. David G. Bear proposes to study the binding of the transcription termination factor rho to specific messenger RNA by following the changes induced in the circular dichroism upon binding. Each co-P.I. will have access to 25% of the total available user time of the instrument. In addition, this proposal contains the contribution of Professor Fritz S. Allen as a user outside of the main user group. Dr. Allen plans to study the molecular mechanisms of allosteric regulation of liver phosphofructo-kinase, by conducting stopped-flow studies and following the CD changes that accompany NAD/NADH conversion.