MULTIWIRE AREA DETECTOR DIFFRACTOMETER

该提案的目的是建立一个高速，X射线衍射 蛋白质晶体学研究数据收集系统 德克萨斯大学奥斯汀分校。 所要求的仪器类似于 MARK II多线区域检测器衍射仪系统由Dr. UCSD的Nguyen Huu Xuong和罗恩哈姆林彻底改变了 收集X射线衍射数据。 已经证明，X射线 大分子系统的衍射数据可以使用这样的 仪器的速率高达100倍， 自动衍射仪 两个区域资源区探测设施目前正在运作， 一个在西海岸的UCSD，另一个在东海岸的弗吉尼亚。 我们提议的设施可以满足四个国家卫生研究院的需要， 德克萨斯大学奥斯汀分校的研究小组，其他四个NIH 支持附近校园的研究项目， 其他机构的其他NIH研究人员可用的时间。 对于如何定位共享资源区域检测器，有许多争论 系统在UT-奥斯汀：PI。的研究小组利用加州大学圣地亚哥分校的设施 三次，并熟悉其操作;实验室人员 已经到位，有资格正确操作和维护 设施的硬件和软件; P.I.的 实验室和现有的旋转阳极可以用作 系统;德州大学奥斯汀校区有三名生物分子晶体学家，多名 其他研究小组都在方便的驾驶距离之内， 几家主要的航空公司都提供前往奥斯汀的航班; UT-奥斯汀有 优秀的机械车间、仪表设计、计算机服务人员 在校园里; UT奥斯汀很可能会获得一台超级计算机，这将是一个 校园内的免费资源对这个用户群有很大的价值;以及 德克萨斯大学奥斯汀分校认识到该设施的价值， 同意为购置区域探测器提供对等资金 X射线衍射仪。

The intent of this proposal is to establish a high speed, x-ray diffraction data collection system for protein crystallographic investigations at the University of Texas at Austin. The instrument requested is similar to the MARK II Multiwire Area Detector Diffractometer System developed by Drs. Nguyen huu Xuong and Ron Hamlin at UCSD that has revolutionized the collection of x-ray diffraction data. It has been demonstrated that x-ray diffraction data for macromolecular systems can be collected using such an instrument at rates up to 100 times that possible using a standard autodiffractometer. Two regional resource area detector facilities are presently in operation, one at UCSD on the west coast and the other in Virginia on the east coast. The facility we are proposing could meet the needs of four NIH supported research groups on the University of Texas at Austin campus, four other NIH supported research programs from nearby campuses, and would still allow time available for other NIH investigators from other institutions. There are many arguments for locating a shared resource area detector system at UT-Austin: the P.I.'s research group has used the UCSD facility three times and is familiar with its operation; laboratory personnel are already in place that are qualified to properly operate and maintain both the hardware and software of the facility; space is available in the P.I.'s laboratory and an existing rotating anode can be used as part of the system; UT-Austin campus has three biomolecular crystallographers, several other research groups are within convenient driving distance, and easy access to Austin is provided by several major airlines; UT-Austin has excellent machine shop, instrument design, and computer service personnel on campus; UT-Austin is likely to acquire a supercomputer which would be a complimentary resource on campus of great value to this user group; and The University of Texas at Austin recognizes the value of this facility and has agreed to provide matching funds for the acquisition of the area detector x-ray diffractometer.