HIGH RESOLUTION DATA COLLECTION OF LARGE OLIGOMERIC PROTEINS

大寡聚蛋白的高分辨率数据采集

• 批准号: 6658538
• 负责人: MARVIN L HACKERT
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658538
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

We propose to collect diffraction data on four projects from our laboratory: ornithine decarboxylase (mODC) from mouse, biosynthetic arginine decarboxylase (bADC) from Escherichia coli (E. coli), the truncated (yeast tE2) components of the pyruvate dehydrogenase complex from Sacchromyces cerevisiae, and the tE2 from the a-ketoglutarate dehydrogenase complex from E. Coli. Data collection of a select group of heavy metal derivatives for both mODC and bADC would aid in the structural determination of these two enzymes.

我们建议收集四个项目的衍射数据， 实验室：来自小鼠鸟氨酸脱羧酶（mODC），生物合成 精氨酸脱羧酶（bADC）来自大肠杆菌（Escherichia coli，E. 大肠杆菌）， 丙酮酸脱氢酶复合物的截短（酵母tE 2）组分 来自酿酒酵母的tE 2和来自α-酮戊二酸的tE 2 脱氢酶复合物。杆菌 选定组的数据收集 mODC和bADC的重金属衍生物将有助于 这两种酶的结构测定。