WIDE-BORE NUCLEAR MAGNETIC RESONANCE SPECTROMETER

请求提供资金，用于购买宽口径200 MHz超导 核磁共振（NMR）光谱仪。 器乐 其能力将与标准口径360 MHz的能力互补 位于爱荷华州大学高场NMR设备的光谱仪。 这种现有的集中式设施是根据BRS要求建模的， 共享使用资源。 博士学位一级核磁共振专家目前 负责维护、升级、咨询、监督 技术人员和操作人员的指导。 广泛的NMR使用基础 目前存在于爱荷华州大学。 添加高功率脉冲 宽口径光谱仪将大大扩大机会， 生物医学研究人员从直接观察整个细胞 代谢过程，以阐明基本的化学反应性 金属酶活性中心模型 大样本量的可用性 (20毫米直径）探头将使多核光谱检查成为可能 生物聚合物（如肝素、血红素蛋白和脂蛋白）， 表现出低溶解度或聚集倾向。 低光谱 天然产物中的氮-15等敏感核， 适用于大直径探头。 各种全细胞和组织 计划进行的研究包括： 培养的哺乳动物细胞，肝素与培养的内皮细胞的相互作用 神经毒素与神经细胞的相互作用，胰岛素受体-胰岛素 培养细胞或膜中的效应物相互作用，以及多核 用潜在的核磁共振成像对比剂处理的组织的弛豫研究 剂. 光谱仪的高功率脉冲能力将是 特别有利于顺磁NMR测量，因为线宽可以 需要非常大和极宽的扫描宽度。 的横 偏振魔角旋转附件将增加新的能力， 固态天然产物和生物聚合物的光谱学。 高 光谱仪的分辨率能力将用于自然 产品鉴定和氟-19观察（我们的 360 MHz仪器）。 除了提供各种新功能之外， 200兆赫频谱仪将大大提高获得高质量 仪器，使得可用仪器时间不是限制性的 研究生产力方面。

Funds are requested for purchase of a wide-bore 200 MHz superconducting nuclear magnetic resonance (NMR) spectrometer. The instrumental capabilities will be complementary to those of a standard-bore 360 MHz spectrometer located in the University of Iowa High-Field NMR Facility. This existing centralized facility is modeled after BRS requirements for a shared usage resource. A Ph.D. level NMR specialist is currently responsible for maintenance, upgrading, consultation, supervision of technicians, and instruction of operators. A broad base of NMR usage currently exists at the University of Iowa. Addition of a high power pulse wide-bore spectrometer would considerably expand opportunities for biomedical researchers ranging from direct observation of whole-cell metabolic processes to elucidation of fundamental chemical reactivity of metalloenzyme active site models. Availability of a large sample volume (20 mm diameter) probe will make possible multinuclear spectral examination of biopolymers (such as heparin, hemoproteins, and lipoproteins) that exhibit low solubility or aggregation tendencies. Spectroscopy of low sensitivity nuclei such as nitrogen-15 in natural products should be practical with the large diameter probe. Various whole-cell and tissue studies are planned including those involving: senescence in anchored cultured mammalian cells, heparin interaction with cultured endothelium cels, neurotoxin interaction with the nerve cells, insulin receptor-insulin effector interactions in cultured cells or membranes, and multinuclear relaxation studies on tissues treated with potential NMR imaging contrast agents. The high power pulse capabilities of the spectrometer would be of particular benefit for paramagnetc NMR measurments, as line widths may be very large and extremely wide sweep widths are required. A cross polarization-magic angle spinning accessory will add new capabilities for spectroscopy of natural products and biopolymers in the solid state. High resolution capabilities of the spectrometer are to be utilized for natural product identification and fluorine-19 observation (not available on our 360 MHz instrument). In addition to providing various new capabilities, the 200 MHz spectrometer would greatly improve accessibility to quality instrumentation, such that available instrument time is not the limiting aspect of research productivity.