ACQUISITION OF GC/MS WITH CI/EI AND FAB SOURCES

埃默里社区目前可用的GC/MS设施没有 有足够的数据处理设施、分辨率和质量范围来处理 目前资助的一系列生物医学项目。的最新进展 质谱学催生了新一代商业仪器 这将使质谱学在生物医学中成为更重要的工具 研究-特别是高分辨率磁扇区系统，它可以 被迅速回收利用。这一设施，与随时可以互换的 来源，提供了研究非挥发性生物分子的机会， 重量大于1000amu的药物等。就像高场 超导核磁共振仪器已经成为生物医学不可或缺的一部分 研究并被马萨诸塞州的非核磁共振专家广泛使用 所要求的类型的光谱仪将在不久的将来得到广泛接受 未来。埃默里的生物医学研究社区已经并将继续 为了扩大规模，超过一半的主要用户最近才来到埃默里。 MS设施不足是目前我们面临的最大限制 在我们的研究项目中。 所要求的仪器将服务于六个主要用户组。这个 仅化学系就会增加两个甚至可能是三个额外的 将成为用户的教职员工。一系列不同的研究计划将是 增强版。其中包括：(A)FAB/MS在研究中的应用 与核酸结合的抗癌药物；(B)研究 荧光化合物在细菌中的分布，(C) 炼油区多环芳香族化合物，(D)制备 标记表面活性剂，(E)抗生素的合成，(F)偶极 环加成化学，(G)含天然丁烯内酯的合成 产物，(H)氧化还原酶机理，和(I)有机钴 与辅酶B12化学相关的化学。 如果获奖，来自VG、芬兰人/MAT和 Kratos将通过我们的样本并通过与用户的讨论进行评估 学术机构中的这些工具。

The current GC/MS facility available to the Emory community does not have adequate data handling facility, resolution, and mass range to deal with the series of biomedical projects currently funded. Recent advances in mass spectrometry have led to a new generation of commercial instruments which will make mass spectroscopy a much more important tool in biomedical research - in particular, high resolution magnetic sector systems which can be quickly recycled. This facility, combined with readily interchanged sources, offers the opportunity to investigate non-volatile biomolecules, drugs, etc. with wieghts greater than 1000 amu. Just as high field superconducting NMR instruments have been an integral part of biomedical research and are used extensively by non-NMR specialists, mass spectrometers of the type requested will gain wide acceptance in the near future. The biomedical research community at Emory has and will continue to expand and over half the major users have come to Emory only recently. The inadequate MS facilities are currently the greatest limitation we face in our research program. The requested instrument will serve a group of six major users. The department of chemistry alone will add two and possibly three additional faculty that will be users. A diverse set of research programs will be enhanced. These include: (a) the application of FAB/MS to the study of anti-cancer drug binding to nucleic acids; (b) the study of the distribution of fluorescent compounds in bacteria, (c) distribution of polynuclear aromatic compounds in oil refinery areas, (d) the preparation of labeled surfactants, (e) syntheses of antibiotics, (f) dipolar cycloaddition chemistry, (g) the synthesis of butenolide containing natural products, (h) oxidation-reduction enzyme mechanisms, and (i) organocobalt chemistry related to coenzyme B12 chemistry. If an award is made, competitive instruments from VG, Finnigan/MAT, and Kratos will be evaluated with our samples and by discussion with users of these instruments in academic institutions.