PURCHASE OF LARGE-SCALE FERMENTATION FACILITY

大学几个不同实验室的研究目标 爱荷华州需要使用大量的电池。项目包括： 1)新型辅酶和甲烷酶的提纯及研究 产生菌，2)产甲烷菌代谢途径的阐明 同时参与生物合成和产生能量的功能，3)X射线 核酸-蛋白质相互作用的结晶学，4)酶 乙醇脱氢酶的结构与功能研究，5)乙醇脱氢酶的调控 酵母基因转录及酵母RNA的结构与功能研究 聚合酶，6)军团病细菌的生理学和7) 药理活性天然产物的生产与生物转化 产品。需要资金用于大规模发酵设施，以 培养出所需数量的微生物。该设施还将是 可供其他大学的合作者使用。许多人 项目与健康有直接关系(例如，军团病和 药理学)和所有这些都是间接相关的。此外，大多数申请者 都在爱荷华州医学院。要用在 拟议的研究几乎涵盖了生物化学的每一个分支学科， 微生物学、遗传学和分子生物学。可供使用的大型 为了合理、安全、高效地发酵，发酵罐设备是必不可少的 多种微生物的制备。此工具将是一个 为大学社区提供持久的资产，并将使 以其他方式无法尝试的各种实验。

The research objectives of several different laboratories at the University of Iowa require the use of large quantities of cells. Projects include: 1) Purification and study of novel coenzymes and enzymes of the methane producing bacteria, 2) elucidation of metabolic pathways in methanogens involved both in biosynthetic and energy producing functions, 3) X-ray crystallography of nucleic acid-protein interactions, 4) enzyme structure-function studies on alcohol dehydrogenase, 5) regulation of transcription of yeast genes, and structure-function studies of yeast RNA polymerase, 6) physiology of the Legionnaire's disease bacterium and 7) production and biotransformation of pharmacologically active natural products. Funds are requested for a large-scale fermentation facility to grow the needed quantities of microbes. The facility will also be available for use by collaborators from other universities. Many of the projects have direct health relatedness (e.g. Legionnaire's disease and pharmacology) and all are indirectly related. Also, most of the applicants are in the College of Medicine at Iowa. The techniques to be used in the proposed studies cover virtually every subdiscipline of biochemistry, microbiology, genetics and molecular biology. The availability of a large fermentor facility is essential for the reasonable, safe and efficient preparation of a variety of microorganisms. This instrumentation will be a lasting asset for the university community, and will make feasible a variety of experiments that could not otherwise be attempted.