HTLV RETROVIRUS INFECTION IN HAITIANS WITH AIDS

获得性免疫缺陷综合症（艾滋病）的爆发正在发生， 海地居民在迈阿密，佛罗里达。 这些艾滋病患者 细胞介导免疫的特征性缺陷与 增加对肺孢子虫等机会性感染的易感性 卡氏疟原虫（PC）、弓形虫和播散性疱疹病毒。 实验室 数据显示，海地艾滋病患者的T淋巴细胞亚群 异常、异常丙种球蛋白血症和循环免疫复合物 类似于艾滋病男同性恋者的症状。 正如已经指出， 男同性恋者艾滋病患者、海地人艾滋病患者有明显增加 卡波西肉瘤（Kaposi's sarcoma，KS） 我们还描述了一种婴儿形式的 13名海地儿童的母亲有明显的免疫缺陷， 异常 最近，其中两名婴儿被发现患有KS 代表了这个国家儿童KS的首次报道。 海地人 也具有相对高的人类T细胞白血病/淋巴瘤发病率 病毒（HTLV）感染（28%），通过血清抗体测定， HTLV特异性细胞表面抗原。 HTLV在海地的存在 男性同性恋人群中HTLV与AIDS的关系 提示HTLV可能在艾滋病的发病机制中起一定作用， 海地人 为了研究该人群中的HTLV感染， 有必要确定检测方法和调查发病机制 艾滋病患者和接触者中的HTLV感染。 现时的建议 用于研究HTLV病毒与宿主的相互作用。 我们将定量HTLV 多肽和核酸序列在外周血白细胞 抗体阳性和阴性的海地人。 患者的组织， 没有艾滋病的人也将在活检和尸检中检查HTLV 材料. HTLV的分离以及已经进行的免疫学研究 现有的HTLV抗体阳性海地血清将提供 患者血清学反应性与 HTLV遗传分化。 对HTLV的免疫应答将在 免疫球蛋白类别、时间表达和病毒体的术语 参与刺激天然免疫的多肽和/或前体。 这些免疫应答将与临床状况相关， 确定潜在的预后因素。 一旦足够敏感 和具体的化验的特点，血清收集自海地人， 迈阿密作为前瞻性多中心CDC协作病例对照的一部分 研究和来自医院和实验室工作人员的证据将被检查 HTLV感染。

An outbreak of acquired immunodeficiency syndrome (AIDS) is occurring in Haitian residents in Miami, Florida. These AIDS patients have the characteristic defects in cell mediated immunity associated with an increased susceptibility to opportunistic infections such as Pneumocystis carinii (PC), Toxoplasm gondii and disseminated Herpesvirus. Laboratory data reveals that Haitian patients with AIDS have T-lymphocyte subset abnormalities, dysgammaglobulinemias, and circulating immune complexes similar to those noted in male homosexuals with AIDS. As has been noted in male homosexuals with AIDS, Haitians with AIDS have a markedly increased rate of Kaposi's sarcoma (KS). We have also described an infant form of AIDS in 13 Haitian children born to mothers with marked immunologic abnormalities. Recently two of these infants have been found to have KS and represent the first reports of childhood KS in this country. Haitians also have a relatively high incidence of human T-cell leukemia/lymphoma virus (HTLV) infections (28%) as measured by serum antibody to HTLV-specific cell surface antigens. The presence of HTLV in the Haitian population and the association of HTLV with AIDS in male homosexuals suggest that HTLV may play some role in the pathogenesis of AIDS among Haitians. In order to study HTLV infection in this population it is first necessary to define detection methods and to investigate the pathogenesis of HTLV infection in both AIDS patients and contacts. The present proposal is for the study of HTLV-virus-host interactions. We will quantitate HTLV polypeptides and nucleic acid sequences in peripheral blood leukocytes from antibody-positive and negative Haitians. Tissues from patients with and without AIDS will also be examined for HTLV both in biopsy and autopsy materials. Isolation of HTLV as well as immunologic studies of already existing HTLV antibody-positive Haitian sera will be undertaken to provide information on the correlation between patient's serologic reactivity and HTLV genetic divergence. Immune responses to HTLV will be measured in terms of immunoglobulin classes, temporal expression and the virion polypeptides and/or precursors involved in stimulating natural immunity. These immune responses will be correlated with clinical conditions to determine potential prognostic factors. as soon as adequately sensitive and specific assays are characterized, sera collected from Haitians in Miami as part of a prospective multicenter CDC collaborative case control study and from hospital and laboratory staff will be examined for evidence of HTLV infection.