ACQUIRED IMMUNODEFICIENCY SYNDROME/ASSOCIATION WITH HTLV

这个项目将研究逆转录病毒之间的联系，特别是 人T细胞淋巴瘤/白血病病毒（HTLV），以及 获得性免疫缺陷综合症（艾滋病）。 系列血清和细胞组织 将从艾滋病患者和那些被认为 有这种疾病的前驱形式。 将测定血清中的HTLV 抗原和针对HTLV病毒或转化细胞的抗体 抗原 任何阳性反应的特异性将通过以下方法确定： 竞争性免疫荧光（IMF）、ELISA和放射免疫沉淀 (RIP)测定。 此外，新鲜的和培养的患者T淋巴细胞和 将分析单核细胞中HTLV蛋白和核酸的存在 酸和用于产生HTLV病毒体。 所采用的技术将 包括：特异性IMF、ELISA、RIP和逆转录酶测定， HTLV蛋白;斑点印迹、Southern和北方印迹以及原位 使用克隆的和非克隆的HTLV核酸探针的杂交测定; 铁蛋白标记HTLV特异性双抗体电镜 测定。 如此测试的细胞还将评估其表型和 使用组织化学染色的功能性质，单克隆细胞表面 标记物和T淋巴细胞辅助或抑制功能测定， 以确定T淋巴细胞的哪个亚类可能与HTLV相关。 经过致死性辐照的新鲜艾滋病细胞和培养的艾滋病细胞将共同培养 与人类和非人类靶细胞系，以研究传播 并从这些患者中分离逆转录病毒。 培养的T淋巴细胞 来自艾滋病患者的病毒将通过故意感染HTLV而永生 从而建立细胞系，并且可以用特异性的 如果艾滋病病原体不完全 与HTLV同源。 从这种转化的艾滋病细胞产生的病毒粒子将 在感染亲本病毒的情况下， HTLV拯救了一种有缺陷的逆转录病毒。 最后是新鲜的培养材料 将注射到灵长类动物体内， 艾滋病样综合征，随后将分析动物的 HTLV的存在。 阐明艾滋病的病原体将是 了解和根除日益致命的公共卫生 问题. 了解HTLV或相关逆转录病毒与 一种非肿瘤性疾病将扩展我们对逆转录病毒毒性的概念， 伙计

This project will study the association between retroviruses, specifically the human T-cell lymphoma/leukemia virus (HTLV), and patients with the acquired immunodeficiency syndrome (AIDS). Serial sera and cellular tissue samples will be obtained from both AIDS patients and those considered to have a prodromal form of the disease. Sera will be assayed for HTLV antigens and for antibodies to HTLV viral or transformed cellular antigens. The specificity of any positive reactivity will be determined by competitive immunofluorescent (IMF), ELISA, and radioimmunoprecipitation (RIP) assays. Further, both fresh and cultured patient T-lymphocytes and monocytes will be analyzed for the presence of HTLV proteins and nucleic acids and for the production of HTLV virions. Techniques employed will include: specific IMF, ELISA, RIP, and reverse transcriptase assays for HTLV proteins; dot blot, Southern and Northern blot, and in situ hybridization assays using cloned and non-cloned HTLV nucleic acid probes; and ferritin-labelled HTLV-specific double antibody electron microscopic assays. Cells so tested will also be evaluated for their phenotypic and functional nature using histochemical stains, monoclonal cell surface markers, and assays for T-lymphocyte helper or suppressor function in order to determine which subclass of T-lymphocytes might be HTLV associated. Lethally irradiated fresh and cultured AIDS cells will be co-cultivated with human and non-human target cell lines to investigate the transmission and isolation of a retrovirus from these patients. Cultured T-lymphocytes from AIDS patients will be immortalized by deliberate infection with HTLV such that cell lines are established and can be examined with specific probes for an AIDS agent in the event that this agent is not completely homologous to HTLV. Virions produced from such transformed AIDS cells will be analyzed for homology to HTLV in the event that infection with parent HTLV rescues a defective retrovirus. Finally fresh and cultured material from AIDS patients will be injected into primates in order to induce an AIDS-like syndrome and the animals will be subsequently analyzed for the presence of HTLV. Elucidation of the etiological agent of AIDS will be an important step in the understanding and eradication of an increasingly lethal public health problem. Understanding the relationship of HTLV or a related retrovirus to a non-neoplastic disease will expand our concept of retroviral toxicity in man.