MOLECULAR CLONING OF PYRUVATE DEHYDROGENASE GENE

我们实验室最近的研究表明，2，3-丁二醇， 在人类酒精血液中发现的两种不寻常的代谢物之一是 主要与遭受痛苦的人类受试者有关 酒精性肝炎或酒精性肝硬变(Casazza等人，酒精 和酒精中毒，支持。1：607-609,1987)。其作用机制是 丙酮酸脱氢酶催化合成2，3-丁二醇 最初由Veech等人提出。(货币。托普。牢房。律例18：151- 179,1981)。根据这份提案，我们刚刚启动了一个项目 丙酮酸脱氢酶(PDH)基因的克隆及鉴定 它的基因根据开发出一种特定的基因探针 人类酗酒的标志。

Recent studies from our laboratory indicate that 2,3-butanediol, one of two unusual metabolites found in human alcoholic blood, is predominantly associated with human subjects who suffer alcoholic hepatitis or alcoholic cirrhosis (Casazza et al., Alcohol and Alcoholism, Suppl. 1: 607-609, 1987). The mechanism of the formation of 2,3-butanediol via pyruvate dehydrogenase was initially proposed by Veech et al. (Curr. Top. Cell. Regul 18: 151- 179, 1981). Based on the proposal, we have just started a project to clone pyruvate dehydrogenase (PDH) gene and to characterized its gene in light of developing a specific probe for a genetic marker for human alcoholism.