IN VIVO NMR STUDIES OF AGING IN CELLS AND ANIMALS

NMR用于动物和细胞衰老的非侵入性研究， 用于研究人类手臂和腿部的年龄变化。 一份生物样本 光谱仪（300/1.9）用于研究动物的年龄变化， 多核光谱学，使用二维NMR和饱和转移 研究代谢率如何随年龄变化的技术。 的 将代谢变化与通过成像研究的形态学变化进行比较 技术，这也是有用的研究变化的分布 药物和代谢物的混合物 设计用于窄孔的探头 Varian XL-200光谱仪用于灌注细胞，如人 不同传代次数的成纤维细胞和不同年龄的供体。 通过多核NMR技术研究细胞的代谢。

NMR is used for the non-invasive study of aging in animals and cells, and for the study of age changes in human arms and legs. A biospec spectrometer (300/1.9) is used to study age changes in animals by multinuclear spectroscopy, using 2-dimensional NMR and saturation transfer techniques to study how metabolic rates of exchange change with age. The metabolic changes are compared to morphological changes studied by imaging techniques, which are also useful for studying changes in the distribution of drugs and metabolites. Probes designed for use with a narrow-bore Varian XL-200 spectrometer are employed to perfuse cells such as human fibroblasts of varying passage and from donors of different ages. Metabolism of the cells is studied by multinuclear NMR techniques.