ROLE OF PDGF EXPRESSSION IN THE NEOPLASTIC PROCESS

猴肉瘤病毒(SSV)的v-sis癌基因编码一种蛋白，该蛋白是 与人PDGF-B或-2链密切相关。C-sis/血小板衍生性 生长因子B基因。V-sis的人类同源物。一直以来都是 进行了克隆和鉴定。当这个基因在基因控制下表达时 逆转录病毒启动子，并导入培养的NIH3T3细胞，它 赋予这些细胞转化的表型。PDGF-B链 已在多种人类胶质母细胞瘤中观察到转录本和 纤维肉瘤。相反，在正常情况下无法检测到此文字记录 胶质细胞或成纤维细胞。所有这些发现以及 人PDGF-B表达产物的免疫学验证 肿瘤细胞支持PDGF-B在肿瘤发生中的作用。我们已经调查了 该生长因子在体内和体外的表达调控。 体内过表达PDGF-B导致人纤维肉瘤的形成 老鼠。这种人类生长因子在不同组织中的低水平表达 组织也是通过将该基因导入到 转基因小鼠。

The v-sis oncogene of simian sarcoma virus (SSV) encodes a protein which is closely related to the human PDGF-B or -2 chain. The c-sis/platelet-derived growth factor (PDGF)-B gene. The human homologue for v-sis. has been cloned and characterized. When this gene is expressed under the control of a retrovirus promoter and introduced in NIH 3T3 cells in culture, it confers the transformed phenotype to these cells. The PDGF-B chain transcript has been observed in a variety of human glioblastomas and fibrosarcomas. In contrast, this transcript is not detectable in normal glial cells or fibroblasts. All of these findings along with the immunological demonstration of the PDGF-B product expressed by such human tumor cells support a role of PDGF-B in tumorigenesis. We have investigated the regulation of expression of this growth factor in vivo and in vitro. Overexpression of PDGF-B in vivo leads to formation of fibrosarcomas in mice. Low levels of expression of this human growth factor in different tissues was also achieved by introducing the gene into the germline of transgenic mice.