3D Printing for World Class Additive Biofabrication

我们需要两件核心设备，以增强我们的世界级添加剂生物制造的能力和能力。这些系统将允许在存在生物细胞的情况下进行3D打印。在目前的设施中，这是极具挑战性和极不安全的--这些系统的采购将使UON和英国成为这一领域的世界领先者，并在国际竞争中领先。添加生物制造是使用添加制造(也称为3D打印)来制造复杂的、受生物启发的结构，在制造过程中存在细胞。这一点很重要，因为生物学是以3D形式存在的，当试图使用支架和类似结构来替换、再生或修复组织时，细胞的存在极大地提高了成功的机会。这项拟议的技术对于支持未来的治疗至关重要，例如器官的恢复或再生，以及毒性新药的筛选，但也准备为世界上一些最大的挑战提供工具和解决方案，包括通过应用于环境科学、生物技术和解决抗菌素耐药性来了解微生物如何与其生态系统相互作用。1.多光子打印机使3D打印能够在纳米级进行，潜在地实现小至100纳米的特征尺寸。我们需要一种具有环境保护的系统，允许在印刷过程中使用生物细胞，这是诺丁汉目前无法实现的重大进步2：微型立体印刷机能够在1-100微米范围内制造，提供互补的制造能力，但也允许更高的生产率，从而有可能在未来进行大规模生产。所需的设备将构成附加生物制造中心的核心，补充由诺丁汉大学资助的系统。3.用于微立体光刻系统的II类外壳，以确保在制造过程中的生物安全保护。关键的是，这些系统将允许用哺乳动物和微生物细胞进行制造。例如，这包括间充质干细胞和角膜干细胞，以创造最终导致治疗干预的组织，但也包括细菌、真菌和藻类，这些细胞可用于生产防污染系统或促进用于合成生物学目的的收获。申请的物品将存放在诺丁汉生物发现研究所的生物打印实验室内。这个环境是一个共享的实验室空间，专门用于在哺乳动物和微生物细胞的制备和使用附近使用添加剂制造和相关技术。该实验室拥有诺丁汉大学添加剂生物制造团队，该团队聚集了一群多学科和多部门的研究人员，他们对使用添加剂制造来指导和控制细胞行为有兴趣和专业知识。这一组包括工程学、生命科学、再生医学和微生物学，旨在了解最终将导致治疗干预或工业生物技术解决方案的结构-功能关系。该小组以再生医学和细胞疗法与诺丁汉大学添加剂制造中心(CfAM)之间的合作为中心，由寻求利用添加剂制造和再生医学之间的接口的国际领先研究人员组成。

We request two pieces of core equipment to augment our capability and capacity for World Class Additive Biofabrication. These systems will allow for 3D printing with biological cells present. This is currently highly challenging and highly unsafe within current facilities - the procurement of these systems will allow UoN, and the UK, to become world leaders in this area and step ahead of the international competition. Additive biofabrication is the use of additive manufacturing (aka 3D printing) to manufacture complex, biologically inspired structures with cells present during the fabrication process. This is important because biology exists in 3D and when attempting to replace, regrow or heal tissues using scaffolds and similar structures, having cells present drastically improves the chances of success. The proposed technology is essential for underpinning future therapies, such as the restoration or regrowth of organs and in the screening of new medicines for toxicity, but is also poised to provide tools and solutions for some of the world's greatest challenges, including understanding how microbes interact with their ecosystem with application to environmental science, biotechnology and addressing antimicrobial resistance.Three items are sought.1: Multiphoton PrinterTo enable 3D printing to occur at the nanoscale, potentially realising feature sizes as small as 100 nm. We require a system with environmental protections that allow for the use of biological cells during printing, a major step forward that is not currently possible at Nottingham.2: Micro Stereolithography PrinterAble to fabricate in the 1-100 micron range, providing complementary manufacturing capability, but also allowing much higher production rates that potentially allow for mass manufacture in the future.The equipment requested will form the centrepiece of the Centre for Additive Biofabrication, complementing systems funded by the University of Nottingham. 3. A class II enclosure for the micro stereolithography system to ensure bio-safety protection during fabrication. Critically, these systems will allow for manufacture with mammalian and microbial cells. This includes for example, mesenchymal and corneal stem cells to create tissues that will ultimately lead to therapeutic interventions, but also bacteria, fungi and algae which can be used to produce systems that are either anti-fouling or promote harvesting for synthetic biology purposes. The requested items will be housed within the Bioprinting Laboratory in the Nottingham Biodiscovery Institute. This environment is a shared laboratory space dedicated to the use of additive manufacturing and related technologies in close proximity to the preparation and use of mammalian and microbial cells. This laboratory houses the University of Nottingham Additive Biofabrication team which brings together a group of multidisciplinary and multi-departmental researchers with an interest and expertise in using additive manufacturing for guiding and controlling cell behaviour. This grouping encompasses engineering, life sciences, regenerative medicine and microbiology and aims to understand the structure-function relationships that will eventually lead to, for example therapeutic interventions or industrial biotechnology solutions. The group is centred on a collaboration between the Regenerative Medicine and Cellular Therapies and the Centre for Additive Manufacturing (CfAM) at the University of Nottingham and is constituted of internationally leading researchers seeking to exploit the interface between additive manufacturing and regenerative medicine.