CLONING AND EXPRESSION OF CAROTENOID BIOSYNTHETIC GENES

转座子标记是一种非常有效的分离方法 编码未知产品的遗传基因座。将使用这种方法 分离玉米和番茄类胡萝卜素相关基因 生物合成途径。类胡萝卜素是一种色素，它发挥着 在植物光保护中的重要作用。类胡萝卜素也是 作为维生素A和维生素A的前体，在人类和动物中必不可少 防止光敏反应的保护剂。类胡萝卜素 生物合成是相当好地理解从生化 分析了许多类胡萝卜素突变体。玉米中的类胡萝卜素突变体， VP-5和番茄中的类胡萝卜素突变体Ghost积累了同样的蛋白质 类胡萝卜素前体，植烯。为了启动对…的研究 基因调控和表达，玉米VP-5基因座将被克隆 通过转座子标记。尽管可转置的元素没有 已在番茄中发现，我们将尝试将克隆的 玉米转座子在工程T-DNA载体上转化番茄的研究 通过感染农杆菌传播。换位 进入番茄幽灵基因座，将被选择并用于克隆 这种基因的。

Transposon tagging is a very powerful approach for isolating genetic loci encoding unknown products. This approach will be used to isolate maize and tomato genes involved in the carotenoid biosynthetic pathway. Carotenoids are pigments which play an important role in photoprotection in plants. Carotenoids are also essential in humans and animals as precursors of vitamin A and protective agents against photosensitive reactions. Carotenoid biosynthesis is fairly well understood from the biochemical analysis of many carotenoid mutants. A carotenoid mutant in maize, vp-5, and a carotenoid mutant in tomato, ghost, accumulate the same carotenoid precursor, phytoene. In order to initiate studies of gene regulation and expression, the maize vp-5 locus will be cloned by transposon tagging. Although transposable elements have not been discovered in tomato, we will attempt to introduce the cloned maize transposable element into tomato on engineered T-DNA vectors to be transmitted by infection with Agrobacterium. Transpositions into the tomato ghost locus will be selected and used for cloning of the gene.