DEVELOP METHODS OF ANALYSIS OF HUMAN COLOSTRUM AND MILK

母乳中的非蛋白氮部分约占25% 在母乳中存在的总含氮化合物中。这 该组分含有所有新发现的生长因子和其他 这些重要液体的生物功能成分。这个项目 将重点放在两个领域： 1)将进行纵向研究，通过以下方式分析已知的乳清蛋白 免疫学方法。糖蛋白将被分离为一组，然后 分离成单独的成分并进行定量。金属蛋白会 被分组隔离、分离和定量。维生素结合蛋白 将会被量化。未知的乳清蛋白将被分离、鉴定 并进行了量化。 2)开发和评价分离NPN化合物的方法。 已知个体NPN的餐后、昼夜和纵向变化 化合物将被测定。未知的NPN化合物将通过 关于氮含量和氮含量的定量的色谱技术 特色化的。

The non-protein nitrogen portion of human milk represents approximately 25% of the total nitrogen containing compounds present in human milk. This fraction contains all of the newly discovered growth factors and other biologically functional components of these important fluids. This project will focus in two areas: 1) A longitudinal study will be performed to analyze known whey proteins by immunological methods. Glycoproteins will be isolated as a group, then separated into individual components and quantitated. Metalloproteins will be isolated in groups, separated and quantitated. Vitamin-binding proteins will be quantitated. Unknown whey proteins will be isolated, identified and quantitated. 2) Methods for isolating NPN compounds will be developed and evaluated. Post-prandial, diurnal and longitudinal variations in known individual NPN compounds will be determined. Unknown NPN compounds will be separated by chromatographic techniques, quantitated with regard to nitrogen content and characterized.