DEVELOPMENT OF DEFINED MEDIUM FOR EMBRYO CELLS

本项目的主要目的是开发一种无血清培养基 适合于原代和早期传代SHE细胞的生长。 这种媒介的出现将促进 遗传毒性测试方案和致癌机制研究， 这两个重要领域都与监管考虑有关， 公共卫生 I期研究包括： (a)通过不同菌株的克隆滴定选择合适的基础培养基 两种或多种候选基础培养基中的血清批次。 (b)生长因子和激素替代血清。 优化 的成分和浓度的克隆滴定。 (c)测定SHE细胞在无血清培养基中的克隆生长反应。 (d)测定血清中SHE细胞的转化生长因子分泌- 通过诱导NRK细胞的贴壁非依赖性生长， 单个TGF-β 1和TGF-β 2鉴定。 (SHE=叙利亚人胚胎）

The primary purpose of this project is to develop a serum-free medium suitable for the growth of primary and early passage SHE cells. Availability of such a medium will facilitate standardization of geneotoxic testing protocols and studies on mechanisms of carcinogenesis, both significant areas relevant to regulatory considerations and the public health. Phase I studies involve: (a) Selection of a suitable basal medium by clonal titration of different serum lots in two or more candidate basal media. (b) Substitution of growth factors and hormones for serum. Optimization of components and concentrations by clonal titration. (c) Determine clonal growth response of SHE cells in serum-free medium. (d) Assay for transforming growth factor secretion by SHE cells in serum- free medium by induction of anchorage independent growth of NRK cells, and identification of individual TGF-beta 1 and TGF-beta 2. (SHE = Syrian Hamster Embryo)