STUDIES OF HUMAN ENDOTHELIAL CELLS OF DIVERSE ORIGIN

目的是研究培养的人的生长和功能。 从不同血管部位分离的内皮细胞(EC) 男性和女性的老年捐赠者。重点将放在审查 增殖潜力、选定的代谢活动、某些运输 功能，以及与止血功能相关的膜特性。 血管组织将从脑死亡的不同部位获得， 心脏跳动的身体，以及大血管和毛细血管EC将 与世隔绝。将生成初级、二级和克隆EC培养物，并 分发到组件项目。 将追求以下目标：确定人的体外寿命 EC；检查EC的细胞遗传学特性；调查和的作用 各种文化成分之间的合作互动，因为它们影响 人内皮细胞在年轻供者来源的二期和三期细胞中的生长 来自老年供者的细胞；测定花生四烯酸的代谢物 由人类EC合成；表征和量化人类EC相互作用 与因子VIII相关的抗原和其他凝血成分；以及 测定人脐静脉内皮细胞囊泡摄取能力和胰岛素结合 检测不同胶原基对内皮细胞功能的影响。

The objective is to study the growth and function of cultured human endothelial cells (EC) isolated from diverse vascular sites in different aged donors of both sexes. Emphasis will be placed on examining proliferative potential, selected metabolic activities, certain transport functions, and membrane properties related to hemostatic function. Vascular tissue will be obtained from different sites in brain-dead, heart-beating cadavers, and large vessel and capillary EC will be isolated. Primary, secondary and cloned EC cultures will be generated and distributed to the component projects. The following goals will be pursued: determine the in vitro lifespan of EC; examine cytogenetic properties of EC; investigate the roles of and cooperative interactions between various culture components as they affect human EC growth in Phase II and Phase III cells from young donors and Phase II cells from older donors; measure the metabolites of arachidonic acid synthesized by human EC; characterize and quantify human EC interaction with Factor VIII-related antigen and other coagulation components; and measure vesicular ingestion capacity and insulin binding in human EC and examine the effect of different collagen substrata on these EC functions.