STRESS & AGING ON THE NEUROPEPTIDES IN PERIPHERAL SENSORY AUTONOMIC SYSTEM

这一提议将检验衰老将改变 P物质和生长抑素两种神经肽的合成和转运 远离感觉神经节，而这种压力会加剧这种影响。Dr。 麦克莱恩开发了一种方法来测量这些物质的积累 多肽在结扎或局部应用秋水仙碱后，已开始 评估麻醉剂和神经操作的影响(通过辣椒素或 VMH损害)在运输中。该项目的第一阶段将包括 急性应激检查及其对迷走神经肽含量的影响 幼年动物的神经、坐骨神经和交感神经节(4 莫斯。-Y)，中年(11个月。-M)或旧(18个月-0)。乙醚和 将使用避震压力。在第二个实验中，慢性 压力(每周5天，连续7个月避免电击，然后是1个月 恢复期)将适用于4个月、11个月或18个月大的动物 年龄和多肽含量的变化将是衡量标准。在第三节 实验，从11个月(M)开始的动物将被用来研究影响 在长达7个月的时间内承受压力，以便获得全日制课程 预期的效果。 在第二阶段的研究中，检查在运输过程中的变化 生长抑素和物质将评估其意义 更改了内容。将使用4个月、11个月、18个月和24个月大的动物。 M组中的动物将被用来确定慢性 强调运输，并从一开始就与内容研究相关 相位。M动物(对照和长期应激)将被用来 测量后肢神经源性炎症反应，以及 分级注射阿司匹林对血浆外渗的影响 P物质。

This proposal will test the hypothesis that aging will alter the rate of synthesis and transport of two neuropeptides, substance P and somatostatin, away from sensory ganglia, and that stress will exacerbate this effect. Dr. MacLean has developed a method for measuring the accumulation of these peptides after ligation or local application of colchicine and has begun to assess the influence of anesthetics and nerve manipulation (by capsaicin or VMH lesions) on transport. The first phase of the project will involve examination of acute stress and its effects on peptide content in the vagus nerve, sciatic nerve and sympathetic ganglis in animals that are young (4 mos. - Y), middle aged (11 mos. - M) or old (18 mos. - 0). Ether and shock avoidance stress will be used. In the second experiment, chronic stress (shock avoidance 5 days/week for 7 months followed by a 1 month recovery period) will be applied to animals beginning at 4, 11 or 18 months of age and changes in peptide content will be measures. In the third experiment, animals begun at 11 months (M) will be used to study the effects of stress in the periods up to 7 months in order to get a full time course of the anticipated effects. In the second phase of study, examination of the alterations in transport of somatostatin and substance will be made to evaluate the significance of the altered content. Animals 4, 11, 18 and 24 months of age will be used. Animals in the M group will be used to determine the effects of chronic stress on transport and correlated with content studies from the first phase. M animals (controls and chronically stressed) will be used to measure the neurogenic inflammatory response in the hindpaw as well as alterations in plasma extravasation in response to graded injections of substance P.